一对双生子卵型鉴定与血瘀证研究  

Identification of Twins and Research of Blood Stasis Syndrome

作　　者：杨杰[1] 丁维俊[1] 王米渠[1] 高锋[2] 刘明[1] 谭从娥[1] 卢圣锋[1] 徐全壹[1] 

机构地区：[1]成都中医药大学,成都610075 [2]四川中医药高等专科学校,绵阳620000

出　　处：《中华中医药杂志》2007年第4期209-213,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技部973项目子课题(No.2003CB517105);国家自然科学基金资助(No.30171126)

摘　　要：目的:以双生子为切入点比较三代家系成员的血瘀证等症状积分,从而为家系的病证结合研究提供新思路。方法:首先采用双生子量表对比双生子性状、形态的相似程度,进行四级评分,即十分、相当、较为、不太分别记3、2、1、0分,并比较两名双生子的指纹图谱,同时进行微卫星DNA基因图谱鉴定。然后用三种量表(血瘀证量表和四诊量表、肾虚量表)对三代家系7个成员进行症状评分。结果:通过卵型鉴定,这对双生子性状、形态大致相似,指纹图谱中10指纹型完全相同,微卫星DNA基因图谱鉴定中大小双15个基因座完全相同,均异于她们的妹妹,因此确认为单卵双生子。经过评分,大小双均为重度血瘀证,而三代家系7个成员中,均表现为虚证积分最高。虽然大小双父母血瘀证分数较低,但大小双与父母均表现为舌下静脉曲张等血瘀证症状因子。结论:三代家系成员症状积分分析显示大小双血瘀证的发病是遗传与环境因素相互作用的结果。Objective： In order to provide new approach for combined of diseases with TCM syndromes, we compared the symptom scores of three generations pedigree by investigating twins. Methods： Applying the scoring table of twins, similar degree of twins＇ character and appearance were contrasted. The seven members of three generations pedigree were given symptom scoring by blood stasis syndrome （BSS） scale, four physical examinations scale and renal deficiency scale to evaluate. At the same time, we compared the finger print of the twins, and identified the twins by microsatellite DNA atlas. Results： By the finger print and microsatellite DNA atlas identification, we may regard the twins as monozygotic twins. The twins were severe BSS by diagnosis. In the seven members of three generations pedigree, they were principally deficient syndrome. Although their parents can not be diagnosed as the BSS, their parents had sublingual varicose. Conclusion： researching syndromes of TCM though analyzing the symptom scoring of three generations pedigree members and monozygotic twins have the promising science value.

关 键 词：双生子 卵型鉴定 血瘀证 

分 类 号：R255[医药卫生—中医内科学]