荷包牡丹碱对抗GABA对大鼠束旁核痛兴奋神经元电活动的影响  

ANTAGONISM OF BICUCULLINE ON EFFECTS OF GABA ON THE ELECTRIC ACTIVITIES OF PAIN-EXCITATION NEURONS IN NUCLEUS PARAFASCICULARIS IN RATS

作　　者：张辉[1] 徐满英[1] 

机构地区：[1]哈尔滨医科大学生理教研室,哈尔滨150081

出　　处：《中国疼痛医学杂志》2007年第2期92-95,共4页Chinese Journal of Pain Medicine

基　　金：黑龙江省科技攻关项目(GC05C40607);黑龙江省教育厅科技研究项目(10551141)资助;医学基础学科青年科学基金资助(060033)

摘　　要：目的:在64只大鼠上,观察了侧脑室(icv)注射γ-氨基丁酸(GABA)后对束旁核(PF)痛兴奋神经元(PEN)电活动的影响。方法:采用电生理学方法,以电脉冲刺激右侧坐骨神经作为伤害性刺激,用玻璃微电极细胞外记录PF内PEN放电。结果:(1)伤害性刺激可使PF内PEN痛诱发放电频率增加;(2)icv注射GABA(500μg/10μl)抑制PEN的电活动,使PEN诱发放电频率减少,潜伏期延长;(3)这种作用可被icv注射GABAA受体拮抗剂荷包牡丹碱(B ic,10μg/10μl)所阻断。结论:icv注射GABA可使大鼠PF内PEN对伤害性刺激的反应减弱,GABAA受体参与介导这种镇痛作用。Objective ： To observe the effects of γ-aminobutyric acid （GABA） on the electric activities of pain-excitation neurons （PEN） in nucleus parafascicularis （PF） in 64 rats. Methods ： The trains of electric impulses applied to the right sciatic nerve were used as noxious stimulation. The discharges of PEN in PF were recorded extracellularly with glass microelectrodes. Results ： （ 1 ） The noxious stimulation increased the frequency of evoked discharges of PEN. （ 2 ） The intracerebroventricular（icv） injection of GABA （ 500 μg/10μl） inhibited the electric activities of PEN, decreased the frequency of evoked discharges and prolongated the latency of PEN. （3） The effects could be blocked by the icv injection of bicuculline （ Bic, 10 μg/10μl） , GABAA receptor antagonist. Conclusion：The response of PEN in PF of rats to the noxious stimulation was weakened by the icv injection of GABA. The GABAA receptor participated in mediating the analgesic effects.

关 键 词：γ-氨基丁酸 荷包牡丹碱 束旁核 痛兴奋神经元 电活动 

分 类 号：R96[医药卫生—药理学]