EB病毒潜伏膜蛋白2的二级结构分析和B细胞表位预测  

Prediction of the secondary structure and B-cell epitopes of EBV LMP2

作　　者：欧琴[1] 朱珊丽[1] 张丽芳[1] 

机构地区：[1]温州医学院微生物学和免疫学教研室,浙江温州325000

出　　处：《温州医学院学报》2007年第2期114-118,共5页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(30671882)。

摘　　要：目的:预测EB病毒(EBV)潜伏膜蛋白2(LMP2)的二级结构和B细胞表位。方法:以EBV标准株(B95.8)LMP2的完整氨基酸序列为基础,采用SOPMA、GOR、nnPredict和HNN四种方法分别预测LMP2的二级结构;并结合其跨膜区域、亲水性、表面可及性、抗原性、极性和柔韧性等进行综合分析,预测EBV LMP2的B细胞优势表位。结果:四种方法对EBVLMP2二级结构的预测均表明,其二级结构中柔性区域以无规卷曲为主,少见转角。B细胞优势表位可能位于其N端199～209、318～322和381～391区段。结论:用多参数预测EBV LMP2的二级结构和B细胞优势表位,可为其单克隆抗体的制备和表位疫苗设计等研究提供理论依据。Objective：To predict the secondary structure and B-cell epitopes of EBV LMP2. Methods： EBV standard strain （B95.8） LMP2 amino acid sequence was selected, The secondary structure was predicted with SOPMA,GOR,nnPredict and HNN.Combing compre-hensive analysis of transmembrane domain,hydrophilicity profile, surface probability,antigenicity index,polarity and average flexibility, B-cell preponderant epitope of EBV LMP2 was further predicted. Results：Using four methods,prediction of the secondary structure of EBV LMP2 indicated that random coils were the main structural type of the flexible region in secondary structure. The B-cell preponderant epitopes were probably in the regions of 199 - 209,318 - 322,381 - 391. Conclusion： Prediction of the secondary structure and B-cell preponderant epitopes of EBV LMP2 may lay the basis for studies of the monoclonal antibody and the design of epitope vaccine.

关 键 词：EBV 潜伏膜蛋白2 B细胞表位 预测 

分 类 号：R373.9[医药卫生—病原生物学]