家兔注射^(125)I-标记重组人表皮生长因子后的药代动力学  

Pharmacokinetics of 125 I labeled recombinant epidermal growth factor after intravenous injection of the drug in rabbits

作　　者：刘秀文[1] 汤仲明[1] 柴彪新[1] 屠敏[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《军事医学科学院院刊》1997年第1期14-16,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：用Ｉｏｄｏｇｅｎ法制备１２５ＩｒｈＥＧＦ，ＳＤＳＰＡＧＥ鉴定放射纯度为（９７．１±０．４５）％。家兔静脉注射１２５ＩｒｈＥＧＦ后８ｈ内血浆总放射性保持在高水平，反相高压液相色谱（ＲＨＰＬＣ）测得１２５ＩｒｈＥＧＦ分量迅速下降，亲水性１２５Ｉ标记物分量增高。１２５ＩｒｈＥＧＦ浓度时间曲线符合二室开放模型，ｔ１／２α和ｔ１／２β分别为９ｍｉｎ和１１．６ｈ。组织浓度梯度为尿＞甲状腺＞肠内容＞肾＞血浆＞胆汁＞颌下腺＞肺。８ｈ内尿排泄（２０．３±５．１）％的注入放射性。I rhEGF was prepared by Iodogen method. Purity was (97.1±0.45)% as determined by SDS PAGE. The results revealed that plasma radioactivities remained at high level within 8 hours, while fraction of 125 I rhEGF determined by RHPLC decreased rapidly and 125 I labeled hydrophilic fraction increased simultaneously. Concentration time curve of 125 I rhEGF was best fitted with two compartment model. Disintegration half lives were 9 min and 11.6 h, respectively. Levels in tissues were urine > thyroid gland > contents in jejunum > kidneys > plasma > bile > submaxillary glands > lungs. A total of (20.3±5.1)% of injected radioactivity was excreted through urine within 8 h.

关 键 词：表皮生工因子 药代动力学 兔 基因 重组 

分 类 号：R977[医药卫生—药品] R969.1[医药卫生—药学]