电针对全脑缺血再灌注大鼠血中一氧化氮、内皮素含量的影响  

Effects of Electroacupuncture on Serum Nitric Oxide and Endothelin Contents in Rats with Cerebral Ischemia-reperfusion Injury

作　　者：王军[1] 范军铭[1] 于震[1] 王玉升[1] 周红艳[1] 

机构地区：[1]河南省中医药研究院

出　　处：《针刺研究》2007年第2期98-101,共4页Acupuncture Research

基　　金：国家自然科学基金资助项目(39270837);河南省自然科学基金资助项目

摘　　要：目的:研究一氧化氮(NO)、内皮素(ET)在全脑缺血再灌注损伤中的作用及电针的保护作用机理。方法:SD大鼠随机分为假手术组、模型组、电针治疗组(取左侧“肩”“外关”“髀关”“足三里”穴)、穴位对照组(取左侧“清冷渊”“灵道”“箕门”“漏谷”穴)、非穴位对照组(取左侧“天泉”与“曲泽”连线中点、“曲泽”与“郄门”连线中点、“五里”与“阴包”连线中点和“膝关”与“中都”连线中点),电针参数为连续波,频率10Hz,波宽0.6ms,电压1.5-3V(以肌肉轻微抽动为度),时间10min。每天1次,共3d。假手术组和模型组只固定不针刺。三动脉结扎法造成大鼠全脑缺血再灌注损伤模型,硝酸还原酶法测定血清NO含量,放射免疫法测定血浆ET含量,原子吸收分光光度计测定脑组织Ca2+含量,干湿重法测定脑组织含水量。结果:与假手术组比较,模型组结扎颈总动脉30min的血清NO含量明显降低,血浆ET含量无明显变化;再灌注30min,血清NO含量显著降低,血浆ET含量显著增加,再灌注120min,脑组织Ca2+含量和含水量显著增加。电针能显著升高再灌注30min后血清NO含量,降低血浆ET含量、脑组织Ca2+含量和含水量,与模型组比较有显著性差异。结论:脑缺血再灌注急性期存在血液NO含量降低而ET含量升高,电针治疗脑缺血再灌注损伤的机制可能与升高血清NO含量和降低血浆ET含量有关。Objective： To observe the effect of electroacupuncture （EA） on serum nitric oxide （NO） and plasma endothelin （ET） contents in cerebral ischemia/reperfusion （CI/R） rats. Methods. Fifty-five SD rats were evenly randomized into sham-operation （sham-op）, model, EA, acupoint-control （acu-con） and non-acupoint （non-acu）-con groups. CI/R model was produced by occlusion of the basilar artery and bilateral common carotid arteries and reperfusion. EA （10 Hz, 1.5-3 V, duration of 0.6 ms） was applied to left ＂Jianyu＂ （LI 15）, ＂Waiguan＂ （TE 5）, etc. for EA group, left ＂Qinglengyuan＂ （TE 11）, ＂Lingdao＂ （HT 4）, etc. for aou-con （being not used for CI in clinic） group, and the mid-points between left ＂Tianquan＂ （PC 2） and ＂Quze＂ （PC 3）, PC3 and ＂Ximen＂ （PC 4）, etc. for non-aou-con group. Carotid venous blood was collected 30 min after CI and after CI/ R respectively for analyzing serum NO content with nitrate reductase （NR） method and plasma ET content with radioimmunoassay, and the brain was taken for detecting its water content and Ca^2＋ content with. flame atomic absorption method. Results： Compared with sham-op group, serum NO contents of model group 30 min after CI and CI/R, that of EA group 30 min after 01, acu-con group 30 min after CI/R decreased significantly （P〈0.05）, and plasma ET content of model group 30 min after CI/R increased considerably （P〈0. 05）. While compared with model group, serum NO content of EA group 30 min after CI/R increased significantly （P〈0.05） and plasma ET of EA group decreased significantly （P〈0.05）. Serum NO level of acu-con group 30 min after CI/R was markedly lower than that of EA group at the same time course. In comparison with sham-op group, Ca^2＋ contents of model group and acu-con group and water cntent ratio of the brain tissue of model group increased significantly （P〈0.05）, while compared with model group, Ca^2＋ content of EA group decreased remarkab

关 键 词：电针 脑缺血再灌注损伤 一氧化氮 内皮素 

分 类 号：R245.97[医药卫生—针灸推拿学]