大豆异黄酮合酶基因的克隆及序列分析  

Cloning and Sequence Analysis of Isoflavone Synthase from Soybean

作　　者：段小瑜[1] 马兵钢[1] 牛建新[1] 

机构地区：[1]石河子大学农学院园艺系,新疆石河子832002

出　　处：《生物技术》2007年第2期3-6,共4页Biotechnology

基　　金：国家自然科学基金项目资助(30460081);新疆维吾尔自治区高等学校科研计划资助项目(FSRPHEXJ)

摘　　要：目的:大豆异黄酮是多酚类混合物,有防治肿瘤发生,提高机体免疫力等多种保健功能。异黄酮合酶(isoflavone synthase,IFS)是合成异黄酮的关键酶。本文为了利用异黄酮的特有生物学功能,从大豆中克隆了该基因。方法:采用PCR扩增从大豆[Glycine max(Linn.)Merr.]总RNA中分离了异黄酮合酶基因,并将其克隆到pUCm-T载体并测序。结果:得到全长1583bp的片段。以期用于构建诱导表达基因敲除系统,并用于无性繁殖植物的无标记基因转化。结论:序列分析表明,异黄酮合酶基因(IFS1)含1583个核苷酸,与已报道的序列比较,核苷酸的同源性为92%。Objective： The soybean isoflavone,a polyphenol mixture,benefits our health care such as tumor prevention and cure,immunity raise.Isoflavone synthase（IFS） is a key enzyme in the synthesis of isoflavone.For making good use of the specific biological function of isoflavone,A cDNA fragment from full length IFS gene was amplified from Glycine max（Linn.） Merr.Methods： A cDNA fragment from full length IFS gene was amplified by polymerase chain reaction from Glycine max（Linn.） Merr.,and it was sequenced after cloned into pUCm-T vectors.Results：A eDNA fragment of 1583bp from full length IFS gene was got. They constructed the induced gene knock out syslem, to use into markers free transgenie plant. Conclusion： The sequencing results indicated that the cloned fragment of ifs 1 contained 1583 nucleotides, and shared a sequence homology of 92% with that from Genbank accession number AFI95798 （IFSI）.

关 键 词：大豆 异黄酮合酶 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学] Q943.2