培养基对透明颤菌生长的影响  

The Influence on Culture Media to Vitreoscilla

作　　者：王洪军[1] 尹旭辉[1] 杨姝明[2] 孙艳[2] 吴益民[1] 张志强[1] 冯立[1] 杨国平[1] 

机构地区：[1]沈阳军区疾病预防控制中心,辽宁沈阳110034 [2]沈阳药科大学中医,辽宁沈阳110016

出　　处：《生物技术》2007年第2期68-69,共2页Biotechnology

基　　金：辽宁省教育厅青年人才基金项目资助(No.05L433)

摘　　要：目的:为了从环境中分离透明颤菌(Vitreoscilla),对培养基和生长条件进行了优化。方法:采用富集培养的方法,对透明颤菌生长的培养基成份进行了筛选试验。结果:在培养温度为32±2℃,50ml/500ml三角瓶,pH 7.8,醋酸钠浓度为0.02%,摇床转速120r/min条件下,透明颤菌生长最佳培养基是:酵母粉0.7%,蛋白胨0.3%。结论:透明颤菌在上述培养基中生长12h可达到高峰。Objective： In order to isolate Vitreoscilla from environment,culture media and growing condition was optimized.Methods： method of cumulation cultivation was used and culture media ingredient was screened with regard to Vitreoscilla.Results： The optimum growth conditions for Vitreoscilla was in medium of yeast 0.7%,tryptone 0.3%,NaAc 0.02%,pH7.8 and 32±2℃,50ml/500ml culture flask,in a shaker incubator set at 120 r/min.Conclusion： When culture media is as aforementioned same,Vitreoscilla reached peak after growth 12h.

关 键 词：透明颤菌 培养基 富集培养 优化 

分 类 号：S476[农业科学—农业昆虫与害虫防治]