骨髓间充质干细胞与血管平滑肌细胞直接接触培养后的分化及超微结构改变  

Study of the effect of direct contact with vascular smooth muscle cells on transdifferentiation and ultrastructure of rat mesenchymal stem cells

作　　者：苗莉[1] 何国祥[1] 景涛[1] 刘建平[1] 蒋清安[1] 江明宏[1] 

机构地区：[1]第三军医大学西南医院心内科,重庆400037

出　　处：《医学研究生学报》2007年第4期374-377,I0004,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30400180)

摘　　要：目的:观察大鼠骨髓间充质干细胞(MSC)与血管平滑肌细胞直接接触培养后向血管成分细胞的分化及超微结构改变。方法:将MSC(DAPI标记)与血管平滑肌细胞按一定的比例混合培养后,用平滑肌细胞抗体SM-α-actin、calponin及内皮细胞抗体CD31免疫荧光染色,并用透射电镜观察MSC的超微结构改变,检测MSC的分化情况。结果:MSC与血管平滑肌细胞混合培养5d后免疫荧光染色,可见细胞核蓝染的MSC与抗SM-α-actin(绿色),抗calponin、CD31(红色)的双标细胞出现;而单独培养的MSC抗SM-α-actin阳性,抗calponin、CD31均为阴性。此外,混合培养后的MSC电镜下可见肌丝状结构。结论:与血管平滑肌细胞直接接触可以诱导MSC向血管成分细胞分化。Objective： To study the effect of direct contact with vascular smooth muscle cells on transdifferentiation and ultrastructure of mesenchymal stem cells （MSC）. Methods：Rat MSC and vascular smooth muscle cells were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with vascular smooth muscle cells for 5 days. MSC were cultured alone as a control. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against smooth muscle a-actin,calponin and CD31. Results ：The MSC cocultured with vascular smooth muscle cells expressed smooth muscle a-actin, calponin and CD31at the fifth day of cultivation, no cells positive for calponin and CD31were detected in the control group;and a lot of filaments were observed in the co-cultured MSC by electron microscopy. Conclusion ：Cell-to-cell contact can induce rat MSC to differentiate into vascular smooth muscle-like cells and endotheliocyte-like cells.

关 键 词：骨髓间充质干细胞 血管平滑肌细胞 共培养 分化 超微结构 

分 类 号：R553.1[医药卫生—血液循环系统疾病]