流体剪应力对EA．Hy926细胞IL-8受体mRNA表达的影响  

Effects of Fluid Shear Stress on the Expression of IL-8 Receptor mRNA in EA.Hy926 Cells

作　　者：余昶[1] 刘肖珩[1] 张怡[1] 

机构地区：[1]四川大学华西医学中心生物医学工程研究室,成都610041

出　　处：《生物医学工程学杂志》2007年第2期303-307,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30570450);四川省青年科技基金资助项目(06ZQ026-009);教育部新世纪优秀人才支持计划(NCET-06-0789)

摘　　要：为研究EA．Hy926细胞在不同时间、不同大小流体剪应力作用下IL-8受体CXCR1、CXCR2 mRNA的表达规律,通过体外培养人脐静脉内皮细胞株EA．Hy926细胞,分别施加5．56、10．02、15．27 dyn／cm2三种剪应力,选取剪切5 min、10 min、15 min、20 min、25 min和30 min、1、2、4和8 h等10个时间点进行观测,以半定量RT- PCR方法检测IL-8受体mRNA表达变化。结果表明,在5．56 dyn／cm2剪应力作用下,与静止组相比,各时间点CXCR1 mRNA与CXCR2 mRNA表达均显著升高(P<0．05)。在10．02 dyn／cm2剪应力作用下,CXCR1 mRNA表达随时间相对缓慢下降;而CXCR2 mRNA表达在30min出现短暂的升高,然后随着作用时间的延长开始缓慢下降。在15．27 dyn／cm2剪应力作用下,其CXCR1、CXCR2 mRNA的表达随时间呈现较显著性降低(P<0．01),当持续作用4 h以上其CXCR2 mRNA表达极低。以上结果表明,不同强度、作用时间的流体剪应力参与调节IL-8受体表达。This study was conducted to elucidate the effects of different fluid shear stress on the expression of IL-8 receptors CXCR1 and CXCR2 mRNA in endothelial cells, EA. Hy926 cells. The HUVEC cell lines were cultured in vitro and then exposed to 5. 56, 10. 02, and 15.27 dyn/cm^2 fluid shear stress respectively for 5min, 10min, 15min,20min, 25min, 30min, lh, 2h, 4h and 8h. Semi-quantitative reversal transcription-polymerase chain reaction （RT-PCR） was used for detecting IL-8 receptors mRNA expression at different times. The results showed that, under 5. 56 dyn/cm^2 shear stress, both the expression of CXCR1 and CXCR2 mRNA increased significantly with time （P〈0. 05）. When exposed to 10.02 dyn/cm^2, the expression of CXCR1 mRNA was down- regulated with time on every occasion. CXCR2 mRNA increased temporally at 30min, then it decreased gradually with time and finally went on at a constant lower level. When exposed to 15. 27 dyn/cm^2, both CXCR1 and CXCR2 mRNA expression decreased significantly with time （P〈0. 01）. These data indicate that the expression of CXCR1 and CXCR2 mRNA of endothelial cell is regulated by fluid shear stress.

关 键 词：流体剪应力 CXCR1 CXCR2 内皮细胞 

分 类 号：R318.01[医药卫生—生物医学工程]