β肾上腺素能受体基因多态性与静息心率的关系  

Association of β-adrenoceptor Gene Polymorphism with Resting Heart Rate

作　　者：牛永红 谢正祥[2] 殷跃辉[3] 

机构地区：[1]清华大学第一附属医院心内科,北京100016 [2]重庆医科大学生物医学工程研究室,重庆400016 [3]重庆医科大学第二附属医院重庆市心律失常治疗中心,重庆400010

出　　处：《生物医学工程学杂志》2007年第2期399-403,共5页Journal of Biomedical Engineering

基　　金：重庆市科委基金资助项目([1999]-18-58);重庆医科大学创新基金资助项目(CX2002020)

摘　　要：探讨β肾上腺素能受体(β-adrenoceptor,β-AR,包括β1、β2和β3三种亚型)基因多态性与静息心率(Resting heart rate,RHR)的关系。用计算机化心电信号分析系统自动测试150例健康受试者(男性80例,女性70例)平卧位的RHR。用聚合酶链反应-限制性片段长度多态性(Polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术和等位基因特异性PCR方法分析β1-AR的Ser49Gly和Arg389Gly;β2- AR的Arg16Gly和Gln27Glu;β3-AR的Trp64Arg基因多态性。β1-AR的Arg389Gly基因多态性与RHR有显著相关性(P<0．05)。而且男性的三种心率间的差异显著性高于女性(P=0．0030 vs 0．0045),其中Gly／Gly基因型受试者具有最大心率(男性:80．98±3．09;女性:84．23±6．28)。β1-AR的Ser49Gly及β2-AR的Arg16Gly和Gln27Glu、β3-AR的Trp64Arg基因多态性与RHR无显著相关性(P>0．05)。RHR与基因型有关,β1-AR的Arg389Gly基因多态性与RHR有显著相关性。男性的三种心率间的差异显著性更高,提示男性的RHR受基因型的影响更明显,从而可在基因水平解释“在男性中心率与死亡率的相关性高于女性”这一临床表象。This study was aimed to shed light on the association of β-adrenoceptor （β-AR,includeing three subgroups：β1,β2 and β3）gene polyrnorphism with resting heart rate（RHR）. The RHRs of 150 healthy subjects （male 80; female 70） in the supine were detected by computerized system for analyzing the electrocardiosignals. Polymerase chain reaction-restrlction fragment length polymorphism （PCR-RFLP） and allele specific-PCR techniques were used to determine Ser49Gly and Arg389Gly polymorphism of β1-adrenoceptor gene; Argl6Gly and Gln27Glu polymorphism of β2-adrenoceptor gene and Trp64Arg polymorphism of β3-adrenoceptor gene; in these subjects. The results were as follows： The Arg389Gly polymorphism of β1-adrenoceptor gene is significantly associated with RHR （P〈0. 05） ,and the difference among three RHRs in male group is significantly higher than in group female group （P= 0. 0030 vs 0. 0045）. Individuals with Gly/Gly genotype have the highest RHR （male：80. 98 ± 3.09 ; female ： 84. 23 ± 6, 28）. No significant association was found among Ser49Gly, Argl6Gly, Gln27Glu and Trp64Arg polymorphism with RHR respectively （P〉0. 05）. RHR is associated with genotype. Arg389Gly polymorphism of β1-adrenoceptor gene is significantly associated with RHRs, and the higher significant difference among male＇s RHRs,when compared with that among female＇s, suggests that male＇s RHRs be more influenced by genotype. Thus the clinical phenomenon of ＂the correlation of heart rate and mortality in male is higher than that in female＂ could be explained at the gene level.

关 键 词：Β肾上腺素能受体 基因多态性 静息心率 

分 类 号：R54[医药卫生—心血管疾病]