中国部分山羊品种线粒体DNAD-loop序列遗传多样性分析  

Analyzing on Mitochondrial DNA D-loop Sequences Genetic Polymorphism of Partial Chinese Goat Breeds

作　　者：刘益平[1] 曹少先[1] 傅泽红[1] 徐敏[2] 刘铁铮[1] 

机构地区：[1]江苏省农科院畜牧研究所,南京210014 [2]四川农业大学动物科技学院,雅安625014

出　　处：《畜牧兽医学报》2007年第4期313-320,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省博士后基金资助

摘　　要：分析了中国部分本地山羊品种(18个品种200个体)以及在中国饲养的引入品种(4个品种25个体)线粒体DNA控制区(D-loop)的全序列,结合已报道的世界其它地方的山羊(15个品种77个体)以及2只野山羊的线粒体DNA控制区(D-loop)全序列共304个体,研究结果表明:山羊线粒体DNA控制区约为1 212 bp,检测到228个变异位点,203种单倍型,单倍型多样度为0.993±0.001;核苷酸多样度0.018±0.001。中国部分山羊的变异类型主要为类型A和B,而在巴基斯坦山羊中还检测到类型C和D。比较分析结果表明中国山羊遗传多样性和基因交流比中国黄牛品种要高。This study analyzed the sequences of mitoehondrial DNA D-loop region of partial Chinese native goat breeds （200 individuals in 18 breeds） as well as 25 individuals in 4 introduced goat breeds, combined the completely D-loop sequences that deposited in GenBank from other regions in the world （77 individuals among 15 breeds and 2 wild goat）. The results showed that there were 1 212 bp length and 228 mutation sites and 203 haplotypes defined in D-loop region for all the 304 individuals, haplotype diversity was 0. 993±0. 001 and nucleotide diversity was 0. 018 ±0. 001, respectively. The Chinese goats were belonging to clades A and B type while the Pakistan goat contained all the clades （A, B, C and D）. It suggested that the Chinese goat breeds had higher genetic diversity and high frequently gene flow among breeds when compared with the Chinese cattle breeds.

关 键 词：山羊 线粒体DNA D-LOOP 遗传多样性 

分 类 号：S827.2[农业科学—畜牧学]