PRRSV和PCV2共感染对猪肺泡巨噬细胞细胞因子IL-10、IL-12p40和IFN-γ mRNA转录的影响  

Effects of PRRSV and PCV2 Co-infection in vivo on the Transcriptions of IL-10,IL-12p40 and IFN-γ mRNA in Porcine Pulmonary Alveolar Macrophages

作　　者：李焕荣[1] 杨汉春[1] 郭鑫[1] 施开创[1] 盖新娜[1] 陈艳红[1] 查振林[1] 

机构地区：[1]中国农业大学农业部预防兽医学重点开放实验室,北京100094

出　　处：《畜牧兽医学报》2007年第4期382-387,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划资助(2005CB523204)

摘　　要：用猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)共感染40日龄健康仔猪,利用实时荧光定量PCR技术对共感染仔猪肺泡巨噬细胞(PAM)细胞因子IL-10、IL-12p40和IFN-γ的mRNA转录水平的变化进行了定量分析。结果表明,IL-12p40 mRNA转录从攻毒后3 d开始显著上调(P<0.05),7 d达高峰,14 d开始下降,但仍显著高于对照组(P<0.05),28 d和42 d低于对照组;IL-10 mRNA在3 d显著上调(P<0.05),14 d达到转录高峰(P<0.01),之后逐渐下降,42 d接近对照组水平;IFN-γmRNA转录水平尽管在3 d和28 d时出现2次转录低峰1、4 d和42 d时出现2次高峰,但只在3 d和7 d差异显著(P<0.05)。由此表明,PRRSV和PCV2共感染可导致PAM细胞因子IL-12p40和IL-10 mRNA的转录在感染早期被激活,而IFN-γ mRNA转录则呈较复杂的动态变化,提示PRRSV和PCV2共感染猪PAM的免疫应答、免疫调节和抗感染功能均受到不同程度的影响。Forty-day-old healthy piglets were co-infected with porcine reproductive and respiratory syndrome virus （PRRSV） and porcine circovirus type 2 （PCV2）. The changes of mRNAs of cytokines in porcine alveolar macrophages （PAM） of the inoculated piglets were analyzed using a real-time fluorescent quantitative PCR （real-time FQ-PCR）. The results showed that the transcriptions of IFN-γ mRNAs exhibited two transcription bottoms at day 3 and 28 post-inoculation and a peak at 7 DPI （days post inoculation） and 42 DPI respectively, with significant differences at 3 DPI and 7 DPI （P〈0.05）. The transcriptions of IL-12p40 mRNAs reached the highest level at 7 DPI, with significant differences at 3 DPI and 14 DPI （P〈0.05）, and then downregulated at 28 DPI and 42 DPI with no significant differences. In addition, the mRNAs transcriptions of IL-10 peaked at 14 DPI with significant differences at 3 DPI （P〈0. 05）, 7 DPI （P〈0.05） and 14 DPI （P〈0.01）, then declined gradually to the level of the control at 42 DPI. Accordingly, the co-in- fection of PRRSV and PCV2 could significantly activate the transcriptions of IL-12p40 and IL-10 mRNAs early during the co-infection, while the transcription of IFN-γ mRNAs witnessed more kinetic changes,which indicat impairments of cellular immune response, immune regulatory and anti-infection function of PAM in dually infected piglets.

关 键 词：猪繁殖与呼吸综合征病毒 猪圆环病毒2型 共感染 肺泡巨噬细胞 细胞因子 

分 类 号：S858.285.3[农业科学—临床兽医学]