Elementary study on the antigenicity of SARS-CoV Mc region  

作　　者：ZU QIONG HU WEI ZHAO WEN BING ZHANG HUI JUN WAN LI FANG JIANG ZHI WEI LIU ZHI HUA LIU BEI GUO LONG 

机构地区：[1]Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou , P. R. China [2]Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou , P. R.China

出　　处：《Journal of Microbiology and Immunology》2006年第3期177-181,共5页中华微生物学和免疫学（英文版）

摘　　要：To test the antigenic activity of M protein （Mc protein） in the inner membrane of SARS-CoV, SARS-CoV Me protein＇s bases locating inside the membrane were cloned, the His-fusion protein was expressed in E. coli and analyzed for its antigenic activity. Among those 7 clinically diagnosed patients＇ sera, there were 5 positive and 2 negative in reaction with His-fusion protein. All of the 20 healthy persons＇ sera and rabbit anti-OC43 and 229E were of negative reaction with His-fusion protein. The animals immunized with His-fusion protein have produced muhi-clonal antibody. The His-fusion protein could specially react with clinically diagnosed SARS patients＇ sera and the animals immunized with His-fusion protein could produce specifically multi-clonal antibody, but it could not react with the sera of healthy persons and the rabbit anti-OC43 and 229E.To test the antigenic activity of M protein (Mc protein) in the inner membrane of SARS-CoV, SARS-CoV Mc protein's bases locating inside the membrane were cloned, the His-fusion protein was expressed in E. coli and analyzed for its antigenic activity. Among those 7 clinically diagnosed patients' sera , there were 5 positive and 2 negative in reaction with His-fusion protein. All of the 20 healthy persons' sera and rabbit anti-OC43 and 229E were of negative reaction with His-fusion protein. The animals immunized with His-fusion protein have produced multi-clonal antibody. The His-fusion protein could specially react with clinically diagnosed SARS patients' sera and the animals immunized with His-fusion protein could produce specifically multi-clonal antibody, but it could not react with the sera of healthy persons and the rabbit anti-OC43 and 229E.

关 键 词：SARS-CoV Membrane protein Prokaryon expression Antigenic activity 

分 类 号：R373.1[医药卫生—病原生物学]