过氧钒烟酸对糖尿病鼠磷酸烯醇型丙酮酸羧化酶的影响  

EFFECTS OF PEROXOVANADATE NICOTINIC ACID ON PHOSPHOENOLPYRUYATE CARBOXYKINASE ACTIVITY IN DIABETIC RATS

作　　者：王艳林[1] 余斌杰[1] 袁敏生[1] 张怡坚[1] 肖亦斌[1] 

机构地区：[1]中山医科大学附属第一医院内分泌科

出　　处：《中山医科大学学报》1997年第1期13-15,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：广东省自然科学基金;博士后基金

摘　　要：用分光光度酶偶联速率法，测定用过氧钒烟酸络合物（ＰＯＶ）和二甲双胍（ＭＥＴ）治疗前后糖尿病鼠肝细胞质的磷酸烯醇型丙酮酸羧化酶（ＰＥＰＣＫ）活性。结果显示：糖尿病鼠肝细胞质的ＰＥＰＣＫ活性较正常鼠明显增加（Ｐ＜０．０１）；ＰＯＶ治疗组经ＰＯＶ治疗后该酶活性明显受抑制（Ｐ＜０．０１），而ＭＥＴ治疗组经ＭＥＴ治疗后该酶活性虽有轻微降低但无统计学意义。ＰＯＶ和ＭＥＴ对正常大鼠的ＰＥＰＣＫ均无明显影响。此结果为ＰＯＶ降低高血糖的分子生化机理提供理论依据，亦为探讨治疗糖尿病新的降糖药提供实验基础。Liver phosphoenolpyruvate carboxykinase (PEPCK) activity of rat with diabetes treated by peroxovanadate nicotinic acid (POV) and metformin was determined by spectrophotometrically enzyme coupling rate method. The results show liver PEPCK activity of diabetic rat's was increased significantly, compared with that of normal rats ( P< 0.01). The rat's PEPCK activity was inhibited obviously after treated with POV for 4 weeks. The enzymatic activity of the diabetic rats treated with metformin was unobviously affected on PEPCK activity of normal rats. The results provided theoretic basis for the molecular biochemical mechanism of POV lowering hyperglycemia, and provided experimental base for exploring a new lowering hyperglycemia drug.

关 键 词：钒酸盐类 糖尿病/酶学 丙酮酸羧化酶 酶抑制 

分 类 号：R977.15[医药卫生—药品] R965.1[医药卫生—药学]