周围神经损伤后外源性神经生长因子及睫状神经营养因子对神经元的保护作用  

PROTECTIVE EFFECT OF EXOGENOUS NERVE GROWTH FACTOR AND CILIARY NEUROTROPHIC FACTOR ON NEURONS AFTER SCIATIC NERVE INJURY IN RAT

作　　者：许家军[1] 何成[1] 宋田斌[1] 赵小林[1] 王成海[1] 姜宗来[1] 路长林[1] 

机构地区：[1]上海第二军医大学解剖学教研室

出　　处：《中华显微外科杂志》1997年第1期32-35,共4页Chinese Journal of Microsurgery

基　　金：国家自然科学基金

摘　　要：目的：研究周围神经损伤后神经生长因子（ＮＧＦ）、睫状神经营养因子（ＣＮＴＦ）对神经元的保护作用。方法：以硅管套接大鼠坐骨神经，局部及皮下分别应用ＳＡＬ（生理盐水）、ＮＧＦ和ＣＮＴＦ。术后２周，应用酶组织化学方法显示相应节段脊髓前角运动神经元和脊神经节感觉神经元中胆碱脂酶（ＣＨＥ）、酸性磷酸酶（ＡＣＰ）酶活性并结合计算机图像分析。结果：与正常侧相比，ＳＡＬ组两类神经元中ＣＨＥ活性显著减弱，ＡＣＰ活性显著增强；ＮＧＦ组感觉神经元两酶活性变化不显著，运动神经元两酶活性有显著变化；ＣＮＴＦ组的结果与ＮＧＦ组相反。结论：周围神经损伤后，外源性ＮＧＦ主要保护感觉神经元，ＣＮＴＦ主要保护运动神经元。Objective:Protective effect of exogenous NGF and CNTF on neurons was studied after peripheral nerve injury.Methods:After silicone tubulization of sciatic nerve in rat, 0 9% saline,NGF or CNTF solution was injected into silicone chamber during nerve repair and subcutis every day after surgery.Enzyme histochemistry was used to show CHE,ACP activity of spinal motoneurons in spinal segments and sensory neurons in spinal ganglions combined with computer image analysis.Results:Compared with the control side,both enzymes activity significantly changed in both neurons in SAL group.Both enzymes activity significantly changed in motoneurons,but not in sensory neurons in NGF group.The results in CNTF group were contrary to that in NGF group.Conclusions:These results suggest that exogenous NGF protects sensory neurons and exogenous CNTF,motoneurons after peripheral nerve injury.

关 键 词：周围神经 损伤 神经营养因子 神经生长因子 

分 类 号：R651.305[医药卫生—外科学] R977[医药卫生—临床医学]