小剂量氢化可的松对脓毒症大鼠早、晚期炎症介质表达的影响  

Effect of low-dose hydrocortisone on early and late cytokine expression in LPS-induced septic rats

作　　者：武志远[1] 曾建生[2] 樊寻梅[2] 

机构地区：[1]广州市儿童医院ICU,510120 [2]首都医科大学附属北京儿童医院ICU,100045

出　　处：《中国小儿急救医学》2007年第2期140-143,共4页Chinese Pediatric Emergency Medicine

摘　　要：目的 研究小剂量氢化可的松（HC）对内毒素（LPS）诱导的脓毒症大鼠早、晚期炎症介质表达的影响。方法 将72只雄性Wistar大鼠随机分为3组：对照组（A组）8只、模型组（B组）32只、小剂量HC干预组（C组）32只。B组和C组设立2、8、16、24h四个时段点，每个时段点各8只（相应的8个亚组用B2、138、B16、B24及C2、C18、C16、C24表示）。腹腔注射LPS（1mg／kg）建立脓毒症大鼠模型，C组腹腔注射LPS后即刻尾静脉注射小剂量HC（6mg／kg），分别在注射后2、8、16、24h分时段采集各组大鼠血清，采用双抗体夹心酶联免疫吸附实验检测各组血清TNF-α和IL-1β含量。采集各组大鼠海马组织5mg，制备细胞总蛋白质，进行蛋白质电泳及蛋白免疫印迹分析，检测高迁移率蛋白（HMG）B1水平。结果 各组血清TNF-α、IL-1β水平均以B2组为高，与A组比较，差异有显著性（P〈0．05），C2组与B2组比较下降明显，差异有非常显著性（P〈0．01）。其余各组TNF-α，IL-1β水平与A组及同时点两两比较，差异无显著性（P〉0．05）。A组海马组织可见微量HMGB1表达；B组在B8表达量始增加，以B16、]324为高（P〈0．05）。B组HMGBl表达有持续增加趋势。C组HMGB1表达较B组呈减少趋势，以C16、C24组减少明显，差异有显著性（P〈0．05）。结论 小剂量HC对早、晚期炎症介质TNF-α，IL-1β、HMGB1的表达有明显抑制作用。Objective To investigate the effect of low-dose hydrocortisone on early and late cytokine expression in lipopolysaccharide（LPS）induced septic rats. Methods Seventy-two Wistar male rats were randomly divided into 3 groups：control group （A group, n -= 8）, model group （B group, n = 32）, low-dose HC treatment group（C group, n = 32）. Both B and C group were subdivided into 4 subgroups according to 2, 8, 16, 24 hr after LPS injection （ n = 8） （ as ]32, ]38, B16, ]324, C2, C8, C16, C24 subgroup）. The septic rat model was established by LPS intraperitoneal injection （ 1 mg/kg）, C group was given by intraperitoneal injection of LPS （ 1 mg/kg） and caudal vein injection of low-dose HC （6 mg/kg）. At serial time points, the animals in each group were sacrificed, serum and hippocampus tissue samples were harvested. Serum levels of TNF-α and IL-1β were measured by the double antibody sandwich ELISA, HMGB1 was analyzed by SDS-PAGE and western blotting. Results Serum levels of TNF-α, IL-1β in B2 group were higher than those in A group （P〈0.05）. TNF-α, IL-1β levels of C2 group were reduced as compared with ]32 group （P 〈 0.05）. TNF-α and IL-1β levels showed no difference among A, B8, B16, B24, C8, C16 and C24 groups （P 〉0.05）. HMGB1 content of hippocampus tissue was expressed slightly in A group, but higher in the B16 and B24 groups （ P 〈 0.05）. B group also showed an increased tendency in HMGB1 expression. HMGB1 content of C group was decreased as compared with B group, especially in C16, C24 group （P〈 0.05）. Conclusion Low-dose HC inhibits the early and late cytokines expression significantly in LPS induced septic rats.

关 键 词：高迁移率蛋白B-1 肿瘤坏死因子-α 白细胞介素-1Β 氢化可的松 内毒素 脓毒症 

分 类 号：R45[医药卫生—治疗学]