AIV H5N1亚型高免卵黄抗体的制备及理化特性研究  

Preparation and Physico-chemical Property of Hyperimmune Yolk Immunoglubulin Against H5N1 Avian influenza virus

作　　者：伍海芽[1] 喻正军[1] 陈剑锋[1] 宋云峰[1] 周明光[1] 杨俊兴[1] 周红波[1] 金梅林[1] 陈焕春[1] 

机构地区：[1]华中农业大学动物医学院华中农业大学农业微生物学国家重点实验室动物病原微生物研究室,湖北武汉430070

出　　处：《动物医学进展》2007年第4期5-8,共4页Progress In Veterinary Medicine

基　　金：国家科技攻关项目(2004BA519A07);973计划(2005CB523003)

摘　　要：应用禽流感灭活疫苗(H5N1,Re-1株)免疫20周龄的高产来航蛋鸡,制备抗AIV H5N1高免卵黄抗体(IgY)。在比较了3种缓冲液对IgY活性的影响后,确定采用水稀释法提取IgY。同时在模拟的胃肠内环境下对IgY的各种理化性质进行了试验。结果表明,纯化的IgY在pH 2.0和胃蛋白酶终浓度为1.5 mg/mL的环境下,以及在pH 8.0和胰蛋白酶终浓度为2.5 mg/mL的环境下,37℃作用1 h后其ELISA抗体效价均无明显下降;同时,热稳定性试验表明,IgY在60℃以下作用30 min,其ELISA抗体效价也无明显下降;中和试验测得IgY的中和效价为1∶640,具有较好的中和H5N1亚型AIV的能力。以上结果表明,本试验所制备的抗禽流感H5N1亚型高免IgY具有较高的抗体效价和中和效价,同时还具有一定的耐蛋白酶消化的作用,可用于H5N1亚型禽流感的预防和治疗。Twenty-week-old egg laying chicken were immunized with inactivated oil emulsion vaccine against H5N1 Avian influenza virus. The water dilution method was choosed as the optimal dilution method by comparing of three kinds of yolk immunoglubulin （IgY） extraction buffer. The purified IgY was stable after some time exposed in pepsin and trypsin and was tolerant of high temperature up to 65 ℃. The titer of virus neutralization antibody was 1 ： 640. These results suggest that the anti-HSN1 AIV IgY could be a new useful biological product for specific antiviral therapy against HSN1 AIV.

关 键 词：AIV H5N1 高免卵黄抗体 理化特性 

分 类 号：S852.659.5[农业科学—基础兽医学]