哺乳动物肠上皮细胞的原代培养  

Primary Culture of Mammalian Intestinal Epithelial Cells

作　　者：刘芳宁[1] 张彦明[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100

出　　处：《动物医学进展》2007年第4期53-57,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30471290)

摘　　要：肠上皮细胞是由多功能干细胞分化成的一个高度组织化系统。从离体的肠组织中分离的肠隐窝单位或肠上皮细胞可在数小时内保持高度活力,但要长期(达到10 d)原代培养肠上皮细胞仍然很困难。文章对肠上皮细胞的分离、鉴定、原代培养所需维持培养基和生长培养基的设计及培养条件等方面进行了综述。Epithelial cells lining the digestive tract represent a highly organized system built up by multipotent stem cells. Long-term primary culture of intestinal cells （up to 10 d） is still difficult despite progress in iso＇ation methodologies and manipulation of the cell microenvironment. In this article, the isolation of viable of epithelial cells,the identification, construction of maintenance and growth media, cell culture conditions were discussed. The main aim is to show the difficulty of primary culture of mammalian intestinal epithelial cells,at the same time , to take some signs or experiences for intestinal epithelial cell culture.

关 键 词：原代培养 哺乳动物 小肠 结肠 上皮组织 

分 类 号：Q813.11[生物学—生物工程]