Fluoro-JadeB染色法显示鱼藤酮致大鼠纹状体和海马神经元退行性病变  

Detection of Rotenone-induced Degenerative Neurons in Striatum and Hippocampus of Rats by Fluoro-JadeB Staining

作　　者：陈峻峰[1] 吴强[1] 刘辉[1] 董兆君[1] 

机构地区：[1]第三军医大学军事预防医学院军事毒理教研室,重庆400038

出　　处：《华南国防医学杂志》2007年第2期1-3,共3页Military Medical Journal of South China

基　　金：国家自然科学基金资助项目(30400347)

摘　　要：目的探讨鱼藤酮慢性中毒致大鼠纹状体及海马神经元退行性病变情况。方法采用微量渗透泵背部植埋的方法,建立大鼠鱼藤酮慢性中毒模型,观察大鼠一般情况,利用Fluoro-jadeB复合荧光尼氏双染法检测纹状体及海马变性细胞和正常细胞的改变情况。结果微量渗透泵植埋后鱼藤酮中毒大鼠出现明显的类似于帕金森病的症状,取脑组织染色,可见纹状体和海马内出现大量的阳性变性细胞,阴性对照组未出现相关改变。结论和传统的鱼藤酮皮下注射法比较,微量渗透泵背部植埋法是建立鱼藤酮慢性中毒模型较为理想的方法。鱼藤酮对纹状体和海马神经元具有显著的损伤作用,可以引起神经细胞发生退行性病变。Objective To study degenerative neurons in striatum and hippocampus of rats induced by chronic rotenone intoxication. Methods The model of chronic rotenone intoxication was established in rats by the subcutaneous implantation of miniosmotic pumps. The general state of the rats were observed,and the degenerative neurons and normal neurons in the striatum and hippocampus were detected by Fluoro- JadeB combined with fluorescent Nissl staining. Results The noticeable syndromes like Parkinson＇ s disease appeared in the rotenone-intoxicateed rats, in whom , there were a number of the positive degenerative neurons in the striaum and hippocampus. The above-mentioned changes were not found in the control group. Conclusions Compared with traditional rotenone subcutaneous injection,osmotic subcutaneous implantation is an ideal way to establish rotenone chronic intoxication model. Rotenone can cause neuronal degeneration in the striatum and hippocampus of rat.

关 键 词：鱼藤酮 海马 纹状体 神经元变性 

分 类 号：R332[医药卫生—人体生理学]