小鼠免疫共刺激信号B7-2 cDNA的克隆与序列测定  

MOLECULAR CLONING OF MURINE COSTIMULATORY SIGNAL B7 2 cDNA

作　　者：王文阁[1] 葛伟文[1] 贺福初[1] 吴祖泽[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《中国免疫学杂志》1997年第1期19-21,共3页Chinese Journal of Immunology

摘　　要：应用反转录ＰＣＲ的技术从小鼠脾细胞中克隆了免疫共刺激信号Ｂ７－２编码区ｃＤ－ＮＡ。小鼠脾细胞经ＬＰＳ刺激２４ｈ后，提取总ＲＮＡ。以总ＲＮＡ为模板进小鼠Ｂ７－２ｃＤＮＡ的反转录ＰＣＲ扩增，获得一特异扩增带，与预期值相符。将扩增的ＤＮＡ克隆于ｐＵＣ１８质粒中，序列测定表明其序列与文献报道一致。B7 2 is a recently identified member of the B7 family.According to the published sequence of murine B7 2.Designed a couple of primers for murine B7 2.Through reverse transcription and polymerase chain reaction,obtained a specific band of mB7 2 cDNA fragment from LPS stimulated murine splenocytes.The obtained fragment was inserted to pUC18 plasmid and transformed to E.Coli.DH5α.Restriction enzyme digestion,PCR and sequencing identified it as murine B7 2 with the whole reading frame of 912 base pairs.

关 键 词：分子克隆 B7-2 序列测定 CDNA PCR 

分 类 号：R392－33[医药卫生—免疫学] Q785[医药卫生—基础医学]