低剂量^(60)Coγ线诱发人血淋巴细胞早熟凝聚染色体畸变的剂量效应曲线研究  

DOSE RESPONSE CURVE FOR HUMAN BLOOD LYMPHOCYTE CHROMOSOME ABERRATIONS INDUCED BY ^(60)CO γ RADIATION OF LOW DOSES USING PREMATURE CONDENSED CHROMOSOME(PCC)

作　　者：冯嘉林[1] 邵松生[1] 林雅萍 刘红珍 邹美君 

机构地区：[1]上海医科大学放射医学研究所

出　　处：《辐射研究与辐射工艺学报》1990年第2期107-110,共4页Journal of Radiation Research and Radiation Processing

摘　　要：本文报道应用人外周血淋巴细胞在离体条件下,用低剂量^(60)Coγ线照射后与M期CHO细胞融合产生G_1-PCC,计数其畸变产额,得到线性剂量效应刻度曲线,Y=0.26+0.028.Dr=0.99,并与常规细胞遗传学方法作了比较,结果表明G_1-PCC方法比常规遗传学方法快速、灵敏。对其作为生物剂量计尚存在的问题也进行了讨论。The human blood was irradiated in vitro by 0-100 10^(-2)Gy. Then thelymphocytes were separated from the whole blood. The mitotic CHO cells fused withlymphocytes. This technique can be used to visualize damage to interphase chromosomeaberrations after irradiation. The frequency of breaks produced in G_1 phase of the ly-mphocytes is scord. The dose-response curve for chromosome fragments was found tobe linear Y=0. 26+0. 028. D, r=0. 99. The method was compared with conventionalcytogenetic. The results presented that the G_1-PCC technique is more rapidly andsensitively. But the G_1-PCC used technique of biological dosimetry has some questionswhich is discussed.

关 键 词：辐射 染色体 淋巴细胞 人体 畸变 

分 类 号：Q345.22[生物学—遗传学]