RAPD用于新生儿病房产气肠杆菌分子流行病学研究  

RAPD Applied to Molecular Epidemiology of Enterobacter aerogenes in a Neonatal Unit

作　　者：李鑫[1] 郑军[1] 安军[2] 宋诗铎[1] 刘瑞霞[1] 蔡英林[3] 

机构地区：[1]天津医科大学第二医院,天津300211 [2]南开大学生命科学学院,天津300071 [3]中国医学科学院,天津300020

出　　处：《中华医院感染学杂志》2007年第4期475-477,共3页Chinese Journal of Nosocomiology

摘　　要：目的建立产气肠杆菌RAPD技术,用于新生儿病房产气肠杆菌分子流行病学研究。方法在具有10张床位的新生儿病房,于同一时间采自4例同期住院患儿分离出5株产气肠杆菌,做RAPD分析及药敏试验,找出流行相关菌株及分析耐药情况。结果两株具有相同RAPD指纹图谱的产气肠杆菌是流行相关菌株,分别采自住院第4天和第10天的两例患儿,在新生儿病房内获得;所有菌株不同程度地对氨基苷类、哌拉西林、三代头孢菌素耐药。结论RAPD技术为产气肠杆菌分子流行病学研究提供了简便、可靠的手段,菌株的耐药情况可能与标本采集之前抗菌药物的应用有关。OBJECTIVE To establish RAPD typing method for Enterobacter aerogenes, and apply RAPD to study molecular epidemiology of E. aerogenes in a neonatal unit. METHODS Five E. aerogenes strains were isolated from four patients in the same neonatal unit at the same time. These strains were typed by RAPD technique. Antibiotic susceptibility was determined by MIC to evaluate drug-resistance. RESULTS Two strains belonging to a unique RAPD-typed ones were epidemiologically related strains. These strains isolated from two patients who hospitalized in the same neonatal unit for four and ten days, respectively. Five E. aerogenes strains were resistant to aminoglycosides, piperacillin and the third-generation cephalosporins in varying degree. CONCLUSIONS RAPD technique is a very easy and reliable molecular tool in the study of E. aerogenes epidemiology. Antibiotic resistance of E. aerogenes is probably related with the history of using antibiotics.

关 键 词：RAPD 新生儿 产气肠杆菌 分子流行病学 分型 

分 类 号：R378.2[医药卫生—病原生物学]