羊抗人IgG和出血热单抗(E_5)在辐射改性的硅橡胶膜上固定化研究  

STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS

作　　者：高金辉[1] 哈鸿飞[1] 张钰华[1] 

机构地区：[1]北京大学技术物理系

出　　处：《辐射研究与辐射工艺学报》1990年第3期163-167,共5页Journal of Radiation Research and Radiation Processing

摘　　要：本文用γ辐射技术,在硅橡胶膜(Silastic)上接枝丙烯酰胺单体(AAM),用不同接枝率的Silastic-AAM膜通过叠氮化后对羊抗人IgG和出血热单克隆抗体(E_5)进行了偶联固定化。同位素^(125)碘标记方法对固定在Silastic-AAM膜上的羊抗人IgG量与接枝率的关系,以及抗体量与其免疫活性的关系进行了测定,得到了适于固定化的抗体偶联反应液最适浓度和最佳接枝率范围;用ELISA法对Silastic-AAM膜固定化的出血热(E_5)单抗进行了免疫活性实验,得到了承某一实验条件下具有明显的阳性反应。结果表明,经适当改进,本法对临床诊断有一定应用价值。In this work films of silicone (silastic) were grafted by monomeracrylamide vis γ-radiation technology and then the ovine anti-human IgG, Epidemichemorrhagic fever (EHF)-MCAb were immobilized. on the silastic-AAM films withdifferent grafting yields passthrough associate reactions. Measurements of relationshipsbetween grafting yields. contents of immobilized antibodies and immunoactivitics forimmobilizcd silastic-AAM films were performed by using ^(125)I method ELISA methodwas used to measure the immunoactivities for the immobilized monoantibody. Theresults showed that the antibodies used can be immobilized on radiation-grafted sili-cone films and this immobilization method has its potential significance in clinicalpractice.

关 键 词：羊抗人IgG 辐射接枝 膜 固定化 

分 类 号：Q503[生物学—生物化学]