氮素对烤烟烟苗蛋白质组分含量、硝酸还原酶活性和干质量积累的影响  

Effects of Nitrogen on the Protein Components Contents,NR Activities and Dry Weight Accumulation of Flue-cured Tobacco Seedlings

作　　者：刘国顺[1] 赵春华[1] 叶协锋[1] 王佩[1] 凌爱芬[1] 

机构地区：[1]河南农业大学国家烟草栽培生理生化研究基地,河南郑州450002

出　　处：《华北农学报》2007年第2期71-74,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家烟草专卖局资助项目(9901007)

摘　　要：选用烤烟K326包衣品种于2006年进行漂浮育苗，研究了氮素对烟苗蛋白质组分含量、硝酸还原酶活性和干质量积累的影响。试验设3个处理，N0（前期氮素胁迫，后期氮素浓度为100mg／L）、N1（前后期氮素浓度均为100mg／L）、N2（前后期氮素浓度均为200mg／L）。结果表明，播种后35～56d，各处理水溶蛋白含量与总可溶蛋白含量变化趋势完全一致，两者呈极显著相关关系，但水溶蛋白含量仅占总可溶蛋白含量的55．6％～79．6％；各处理醇溶蛋白含量变化呈单峰曲线，峰值出现在播种后49d；播种后42—56d，不同处理碱溶蛋白含量表现为：N2〉N1〉N0。氮素浓度过大反而降低烤烟叶片NR活性，不利于烟苗的氮素代谢；播种后35～56d，3个处理根干质量均表现为N1〉N2〉N0，而N1，N2叶干重均大于N0。The effects of nitrogen on the contents of protein components, NR activities and dry weight accumulation in the seedlings of flue-cured tobacco K326 in the floating system were studied in 2006. The experiment involved three treatments： N0： nitrogen was not applied in early stage and 100 mg/L nitrogen was applied in later stage; N1： 100 mg/L nitrogen was applied in both stages; N2：200 mg/L nitrogen was applied in both stages. The results indicated that during 35 - 56 days after seedling, the changing tendency of water-soluble protein content for each treatment was the same as that of total soluble protein content, the correlation between which was highly significant. The content of water- soluble protein accounted for 55.6 % - 79.6 % of total soluble proteins. The changes of alcohol-soluble protein content of each treatment presented a single-peak curve and the peak appeared on the 49th day after seedling. The contents of alkali-soluble proteins among the three treatments. Took on the tendency of N2 〉 N1 〉 N0 after seedling 42 d. N2 decreased the NR activities. The root dry weights in the three treatments were in order of N1 〉 N2 〉 N0, while the leaf dry weights of both N1 and N2 were greater than that of N0.

关 键 词：氮素 烟苗 蛋白质组分 硝酸还原酶 干质量 烤烟 

分 类 号：S572.01[农业科学—烟草工业]