适于SSR分析的大豆干种子中DNA快速提取  

Rapid Extraction of Genome DNA from Soybean Dry Seed for SSR Analysis

作　　者：张伟[1] 谢甫绨[1] 宋显军[1] 曹萍[1] 郭玉华[1] 

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110161

出　　处：《华北农学报》2007年第2期133-135,共3页Acta Agriculturae Boreali-Sinica

基　　金：国家"948"计划项目(2003-Q04)

摘　　要：为了探寻大豆干种子中的DNA快速提取方法，以2个大豆品种的干种子为材料，在SDS提取液中加入蛋白酶K、NP-40、Tween-20情况下，并减少氯仿／异戊醇的抽提次数，研究对提取DNA质量的影响。结果表明，SDS提取液中加入3种试剂后，即使使用氯仿／异戊醇抽提1次，其DNA的0D260／0D280值也在1．8—2．0之间，能够满足SSR扩增模板的需求。说明此方法既可获得较高纯度的DNA，又可大大缩短大豆干种子中的DNA提取时间。To develop a rapid DNA extraction method, the quality of DNA was examined with soybean dry seeds as materials, adding proteinase K, NP-40 and Tween-20 to SDS extracting solution, and reducing the chlorofonn/isoamyl extraction times. The results showed that when adding the three reagents to SDS extracting solution, the OD260/OD280 value of DNA was between 1.8 and 2.0 even extracted once by chlorofonn/isoamyl. Thus, good quality and quantity template DNA suitable for SSR analysis was obtained with reduced time.

关 键 词：大豆 DNA提取 SDS SSR 

分 类 号：S565.1[农业科学—作物学]