反义肽核酸技术在临床医学中的应用研究现状  

The current research of antisense peptide nucleic acid technique in clinical application

作　　者：颜艳[1] 李兰英[2] 

机构地区：[1]天津医科大学病理生理教研室,天津300070 [2]天津医科大学代谢病医院内分泌研究所,天津300070

出　　处：《国际内科学杂志》2007年第4期247-248,F0003-F0004,共4页International Journal of Internal Medicine

基　　金：国家自然科学基金资助项目(30370682);天津市科技发展计划基金资助项目(05YFGDSF02700)

摘　　要：肽核酸是一核酸类似物,是核酸中的糖-磷酸主链被一合成的肽链所取代,最终形成一种非手性的、不带电荷的类似物。与核酸分子相比,肽核酸有更高的稳定性、碱基特异性。配体-受体、连接带正电荷基团如赖氨酸或精氨酸残基或核定位信号肽等可以提高肽核酸的通透性。肽核酸在分子生物学和诊断学域有广泛应用。活体外研究显示肽核酸可以抑制目的基因的转录和翻译,因此其反义形式也可应用于临床疾病的治疗。Peptide nucleic acid（PNA） is a nucleic acid analog in which the sugar phosphate backbone of natural nucleic acid has been replaced by a synthetic peptide, resulting in an achiral and uncharged mimic. It is chemically stable and basio-specific compared with nucleic acid. Ligand-receptor, linking positive charge groups such as lysine or arginine residue or nuclear localization signal peptide（NLS） can all improve its permeability. Peptide nucleic acid has been generally used in molecular biology and diagnostics. Results of in vitro studies indicate that PNA could inhibit both transcription and translation of targeted genes, so its antisense form is also used in the therapy of clinical diseases.

关 键 词：肽核酸 反义肽核酸 反义技术 

分 类 号：R453.9[医药卫生—治疗学]