FT-SERS研究非极性R侧链氨基酸在银胶体系中的吸附状态  

Study on Adsorption of Nonpolar R-Side Amino Acids on Silver Nanoparticles by FT-SERS

作　　者：黎司[1] 周光明[1] 杨大成[1] 虞丹尼[1] 彭红军[1] 伍辛军[1] 王宁[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《光谱学与光谱分析》2007年第4期711-715,共5页Spectroscopy and Spectral Analysis

基　　金：西南师范大学校青年基金(SWNUQ:2005008)资助

摘　　要：利用傅里叶变换表面增强拉曼光谱（FT-SERS）研究了以L-蛋氨酸为代表的非极性R侧链氨基酸在纳米银衬底上的吸附状态和相互作用的特性，并结合浓度、pH值的变化探讨了吸附作用的特点和规律。实验结果表明，L-蛋氨酸在银胶上的最佳FT-SERS的浓度范围为10^-3～10^-4mol·L^-1；其pH范围以酸性、等电点、碱性而分段，当pH值在等电点附近及酸碱度过大时的FT-SERS都较差；L-蛋氨酸与纳米银的作用是通过氨基、羧基、硫与银的物理、化学吸附，其吸附态随着pH值的变化而改变。结合文献，对非极性R侧链氨基酸与纳米银的吸附特性作了总结，以期有助于对氨基酸、蛋白质、多肽、酶等相关领域更深入的研究。The adsorption orientation and adsorption characteristics of aliphatic-nonpolar amino acids represented by L-methionine on silver nanoparticles were studied by FT-surface-enhanced Raman spectroscopy （FT-SERS） method with different pH and concentrations. According to the experiments, in order to get the best FT-SERS effects, the optimal concentration range of the L-methionine on silver sol is between 10-3 mol · L^-1-10^-4 mol · L^-1. The FT-SERS effects and adsorption model change with pH; The SERS are poor when pH is near the isoelectric point or over 11 or 3. The interaction of L-methionine with Ag nanopartides is the physical and chemical adsorption by NH^3, COO^-, S, which could also be effected by pH. From the results and previous reports on this aspect, the general rules of the adsorption model and the orientation of the aliphatic-nonpolar amino acids on silver nanoparticles were speculated, which would be useful for the study of amino acids, protein, peptide, enzyme etc.

关 键 词：FT-SERS 非极性R侧链氨基酸 蛋氨酸 浓度 PH值 吸附 

分 类 号：O657.3[理学—分析化学]