Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb^(3+)-tiron complex as a fluorescence probe  

作　　者：LV Xue-fei ZHAO Yi-bing ZHOU Qun-fang JIANG Gui-bin SONG Mao-yong 

机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beifing 100085, China [2]Department of Chemistry, the Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China

出　　处：《Journal of Environmental Sciences》2007年第5期616-621,共6页环境科学学报（英文版）

基　　金：Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2006AA06Z424); the National Basic Research Program (973) of China (No. 2003CB415001);the Pilot Project of Knowledge Innovation Program of Chinese Academy of Sciences (No. KJCX2-SW-H06).

摘　　要：A sensitive method based on the fluorescence quenching effect of the Tb^3＋-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus （ALP） released from fish plasma. The detection limit was 5.4 ng/ml （S/N=2）, and the relative standard deviation of the quenching effect （6 replicates） was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.A sensitive method based on the fluorescence quenching effect of the Tb^3＋-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus （ALP） released from fish plasma. The detection limit was 5.4 ng/ml （S/N=2）, and the relative standard deviation of the quenching effect （6 replicates） was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.

关 键 词：alkali-labile phosphoprotein phosphorus （ALP） Chinese loach fluorescence quenching Tb^3＋-Tiron complex VITELLOGENIN 

分 类 号：X835[环境科学与工程—环境工程]