云南鹅群体遗传结构的随机扩增多态DNA分析  

Analysis of Population Genetic Structure of Yunnan Goose Using RAPD Markers

作　　者：王磊[1] 霍金龙[1] 苗永旺[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《云南农业大学学报》2007年第2期265-269,共5页Journal of Yunnan Agricultural University

基　　金：云南省教育厅科学研究基金项目资助(5Y0196B)。

摘　　要：采用随机扩增多态性DNA标记技术对云南鹅主产区6个不同地点的45个个体进行了群体遗传学分析,从85个随机引物中筛选出的23个引物对所有个体的总基因组DNA进行了PCR扩增,共检测出178条DNA片段,其中150条属多态性片段(多态率84.27%),计算了各座位在群体中的基因频率、杂合度和群体Shannon多样性指数等参数。结果表明:云南鹅的群体遗传变异较丰富。In this study, random amplified polymorphic DNA （RAPD） technique was used to analysis the genetic diversity of 45 samples from 6 different areas in the hometown of the yunnan goose. 23 polymorphic primers which had been screened out from 85 primers were used to amplify the total genome DNA of 45 yunnan gooses, 178 bands were assayed, of which 150 bands （84.27%） were poly- morphic. The allele frequencies at each locus, genetic heterozygosity, Shannon index of genetic diversity had been calculated. The results indicated that there is rich variation in the population of Hunan goose.

关 键 词：云南鹅 遗传多样性 RAPD 杂合度 SHANNON多样性指数 

分 类 号：S835.2[农业科学—畜牧学]