砂海星酸性粘多糖的分离纯化  

Isolation and Purification of Acid Mucopolysaccharide from Luidia quinaria von Martens

作　　者：潘广昌[1] 郭承华[1] 刘传琳[1] 董新伟[1] 

机构地区：[1]烟台大学化学生物理工学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2007年第2期108-111,共4页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：烟台市科学技术发展计划项目(2006222)

摘　　要：利用碱提法结合酶解法从砂海星中提取酸性粘多糖.粗品经三氯乙酸和二次酶解除蛋白后,用42%和80%的乙醇分级沉淀分别得到多糖Ⅰ和多糖Ⅱ,其总糖含量分别为71.6%、80.4%,蛋白质含量分别为1.52%和1.38%.多糖Ⅱ依次用离子交换层析(DEAE-52)和葡聚糖凝胶(G-50)进行分离、纯化,结果表明分子中带有比较均一的电荷,硫酸基离子鉴定反应为阳性,并且组分比较单一;用葡聚糖凝胶(G-100)对其分子量进行测定,其平均分子量约为12kD.Acidic mucopolysaccharides of Luidia quinaria yon Martens are extracted by sodium-hydroxide method and enzyme-decomposition method. The gross products are deproteined by trichloroacetic acid and twice enzymic hydrolysis. The purified product solution is then precipitated in succession with ethanol （42% and 80% ） to get acidic mucopolysaccharide fractions Ⅰ and Ⅱ , of which the contents of total sugar are 71.6% and 80.4% while the contents of protein are 1.52% and 1.38% ,respectively. Fraction Ⅱ is further separated and purified by ion-exchange chromatography （DEAE-52） and Sephadex （G-50） and further analyzed with Sephadex （G-100）. The results show that fraction Ⅱ are composed of one components with sulfate. The average molecular weight of this acidic mucopolysaccharide is about 12 kD.

关 键 词：砂海星 酸性粘多糖 分离 纯化 

分 类 号：TQ027[化学工程]