磺胺甲恶唑酶联免疫分析法的建立  

The Establishment of a Competition Inhibition ELISA for Sulfamethoxazole

作　　者：冯婷婷[1] 李子颖[1] 刘一兵[1] 许文革[1] 官国英[1] 韩世泉[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《同位素》2007年第1期20-23,共4页Journal of Isotopes

摘　　要：建立了磺胺甲恶唑（Sulfamethoxazole，SMX）酶联免疫分析方法，用以检测动物源性食品中SMX的残留。利用辣根过氧化物酶（HRP）标记SMX，兔抗SMX抗体制备固相抗体，通过标本中的SMX和一定量酶标SMX竞争结合固相抗SMX多抗，标本中SMX的量和显色后的OD450呈负相关的原理检测标本中的SMX含量。经方法学鉴定，本方法的灵敏度为0．067μg／L，批内变异系数〈10％，批间变异系数〈20％。牛奶、肉类、鸡蛋、蜂蜜样品的回收率分别为94．9％～116．0％、82．0％～107．6％、81．6％～94．9％、89．0％～93．0％，符合免疫分析方法的要求。The preparation of horseradish peroxidase labeled sulfamethoxazole（SMX）and polyclonal antibody coated plates are described. The principle for the measurement of SMX is that the SMX in the sample and the enzyme-labeled SMX bind competitively the polyclonal antibody against the SMX in the solid phase, and the signal falls with the increasing SMX concentration. The sensitivity of the assay is 0. 067 μg/L. The intra-and interassay CV are below 10% and 20% respectively. The analytical recoveries rate of milk, meat, egg and honey is 94.9%-116.0%, 82.0%-107. 6%, 81.6%-94. 9% and 89.0%-93.0% respectively. A sulfamethoxazole-specific ELISA is established to analyze SMX residues in animalbased food products.

关 键 词：磺胺甲恶唑(SMX) 酶联免疫分析(ELISA) 酶标抗原 

分 类 号：R392-33[医药卫生—免疫学] Q814.9[医药卫生—基础医学]