单次化疗后肿瘤内^99Tc^m-Annexin V的分布与bcl-2、bax蛋白表达的相关性研究  

Distribution of ^99Tc^m-rh-Annexin V in Tumor and Expression Relationship of bcl-2, bax After a Single Dose of Chemotherapy

作　　者：张欣[1] 李亚明[1] 张延军[2] 季晓鹏[3] 陶莉[2] 张剑英[4] 赵贞贞[4] 朱毅[2] 杨春[2] 赵明[3] 田爱娟[3] 

机构地区：[1]中国医科大学附属第一医院核医学科,辽宁沈阳110001 [2]大连医科大学附属第一医院核医学科,辽宁大连116011 [3]大连医科大学附属第二医院核医学科,辽宁大连116023 [4]北京师范大学化学学院放射性药物教育部重点实验室,北京100875

出　　处：《同位素》2007年第1期36-40,共5页Journal of Isotopes

摘　　要：采用^99Tc^m-rh-Annexin V作为核素凋亡显像示踪剂，观察小鼠单次化疗后肿瘤内bcl-2与bax蛋白的表达。结果显示，单次化疗后化疗组肿瘤组织的放射性摄取百分数（％／g）、阳性细胞数及bax蛋白表达均明显多于对照组（P〈0．01）；bcl-2蛋白表达两组间差异无显著性（P=0．220）；化疗组bcl-2／bax明显高于对照组（P〈0．01）。相关性研究表明，对照组与化疗组肿瘤组织的放射性摄取百分数与TUNEL阳性细胞数均呈明显正相关（r=0．801，r=0．769）。对照组肿瘤组织内bax蛋白表达与放射性摄取及TUNEL阳性细胞数均呈明显正相关（r=0．849、0．652），bcl-2／bax比值与放射性摄取百分数及TUNEL阳性细胞数均呈明显负相关（r=-0．820、-0．694）。以上结果提示，肿瘤组织内^99Tc^m-rh-Annexin V的分布可以反映化疗后早期肿瘤组织细胞凋亡的状况以及凋亡调控蛋白bax表达水平的变化。The expression of bcl-2 and bax after the single dose of chemotherapy with ^99 Tc^m- rh-Annexin V as the tracer of tumor apoptosis imaging is studied. Tumor cell apoptosis is examined by TUNEL methods, and the expression of bcl-2 and bax in tumor are determined by immunohistochemical methods. Single dose of chemotherapy significantly increased the tumor uptake of ^99Tc^m-rh-annexin V and the positive number of TUNEL, as well as the expression of bax （P〈0.01）. The expression of bcl-2/bax is significantly higher in contrast group than in therapy group （P〈0.01）. The radio-uptake in tumor correlates well with the positive number of TUNEL in contrast group and therapy group（r=0. 801, r=0. 769）. In therapy group, the expression of bax correlates well with the uptake and the positive number of TUNEL（r=0. 849, r=0. 652）, but the bcl-2/bax correlates negatively with radiouptake and the positive number of TUNEL（r=-0. 820, r=-0. 694）. The distribution of ^99Tc^m-rh-annexin V in tumor reflectes not only the degree of apoptosis of tumor cells, but also the change of bax expression after the single dose of chemotherapy.

关 键 词：^99Tc^m-Annexin V bcl-2 BAX 表达 相关性 

分 类 号：R817-33[医药卫生—影像医学与核医学] R817.9[医药卫生—放射医学]