β纤维蛋白原基因-455nt G/A多态性与急性心肌梗死的关系研究  

Research of association between βFibrinogen gene-455nt G/A polymorphism and acute myocardial infarction

作　　者：余元勋[1] 王爱玲[2] 陈森[2] 朱霖[1] 孙国梅[1] 

机构地区：[1]安徽省优生优育遗传医学中心安徽医专,230061 [2]安徽医科大学附院心内科

出　　处：《中国优生与遗传杂志》2007年第4期21-22,25,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的通过检测急性心肌梗死患者及对照者β纤维蛋白原基因-455ntG/A多态性,探讨该多态性与急性心肌梗死发病的关系,寻找急性心肌梗死发病的遗传性危险因素。方法β纤维蛋白原基因-455nt多态性分析:(1)碘化钾法提取人基因组DNA;(2)多聚酶链反应(PCR)扩增目的基因片段再用HaeⅢ限制性内切酶消化;(3)酶切产物经3%琼脂糖凝胶电泳分离紫外灯下检测结果;(4)SPSS11.5软件分析数据。结果(1)急性心肌梗死组中β纤维蛋白原基因-455ntA等位基因频率显著高于对照组。(2)急性心肌梗死组中β纤维蛋白原基因-455ntGA和AA基因型携带者频率显著高于对照组。(3)多元logistic回归分析发现,性别、血糖及β纤维蛋白原基因-455ntAA+GA基因型对急性心肌梗死的发病有明显影响。结论(1)β纤维蛋白原基因-455ntA等位基因可能是急性心肌梗死发病的遗传性危险因素。(2)急性心肌梗死发病与性别、血糖水平有关。Objectives： To analyze the frequency of β -fibrinogen gene -455nt G/A genotypes in acute myocardial infarction （AMI） group and control group, and TO investigate wether the -455nt G/A polymorphisms were associatied with the risk of AMI. Methods： The -455nt polymorphisms in the βfibrinogen gene were detected by polymerase chair reaction amplication of fragments of the DNA. Polymerase chain reaction products were subjected to 3 hours digestion by 4U Haelll restriction enzymes and were then separated using 3% agarose gel electrophoresis and visuslized by UV light. Interactions between these changes and other factors were analyzed with SPSS statistical software 11.5. Results ： （ 1 ） The frequences of - 455nt A allele were higher in AMI group than in control group. （2） The frequences of -455nt AA and GG genotypes in AMI group were higher than that in control group, （3） The levels of sugar and tfiglyceride in blood were higher in the patients than that in the control subjects. （4） βfibrinogen -455nt GA and AA genotypes were contributed to acute myocardial infarction. Conclusion： （1） Fibrinogen β-455nt G/A polymorphisms were a genetic risk factor of acute myocardial infarction, （2） Sex, blood sugar and triglyceride level had associations with AMI.

关 键 词：急性心肌梗死 β纤维蛋白原基因 基因多态性 

分 类 号：R542.22[医药卫生—心血管疾病]