α-乙酰乳酸脱羧酶的磁性固定化条件研究  

Immobilization of α-Acetolactate decarboxylase with magnetic polyvinyl alcohol

作　　者：臧姝[1] 钱斯日古楞 王红英[1] 崔茂欣[1] 

机构地区：[1]大连轻工业学院生物与食品工程学院,辽宁大连116034

出　　处：《大连轻工业学院学报》2007年第1期15-17,共3页Journal of Dalian Institute of Light Industry

基　　金：大连市科技攻关项目(2004B3SF175)

摘　　要：采用分散聚合法,在Fe3O4磁流体存在下,通过PVA分子单体共聚制备出磁性聚乙烯醇微球.微球粒径分布在2.5×10-2～5×10^-2 μm,其中3.7×10-2～4.1×10^-2 μm的微球占总微球的44%,制备微球粒径分布均匀.以磁性聚乙烯醇微球为载体,通过戊二醛交联法进行ALDC的固定化,制备固定化酶,并对其固定化条件进行了初步研究.结果显示,在酶固定化过程中,自由酶的添加量为20 mL/g微球,戊二醛的添加量为0.98%.在其固定化最佳条件下,制备的固定化ALDC的活力为65 180 U/g,而且其比活、活性回收率分别可达872.32 U/mg和42.33%.α-Acetolactate decarboxylase（ALDC）; magnetic microsphere; enzyme immobilization Abstract：Magnetic microspheres were prepared and the diameter was 2. 5 × 10^-2- 5 × 10^-2μm in which the microsphere with 3.7 × 10^-2 -4.1 ×10^-2μm in diameter was about 44%. α-Acetolactate decarboxylase was immobilized in magnetic microsphere by linking with glutaraldehyde. The results showed the optimum immobilized α-Acetolactate decarboxylase being 20 mL/g the magnetic microspheres, 0. 98% glutaraldehyde, in which the activity of immobilized α-Acetolactate decarboxylase was 65 180 U/g, the specific activity was 872.32 U/mg and the activity recovery was 42.33%.

关 键 词：α-乙酰乳酸脱羧酶(ALDC) 磁性微球 酶固定化 

分 类 号：Q814.2[生物学—生物工程]