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机构地区:[1]河南师范大学生命科学学院,河南新乡453007
出 处:《广西植物》2007年第2期250-254,共5页Guihaia
基 金:河南省重点科技攻关项目(0423032100)~~
摘 要:研究PP333对怀地黄试管苗生长及一些生理指标的影响。通过单因子实验、比色法和愈创木酚法探讨PP333对试管苗的影响。结果表明,不同浓度PP333均促进试管苗芽的萌发,使根系粗壮,根数增加,低浓度PP333(0.01、0.05mg·L-1)促进试管苗茎的伸长生长,高浓度(0.1、2mg·L-1)抑制茎、叶生长,PP333浓度为2mg·L-1时壮苗效果最佳。PP333处理使试管苗生长中期叶片可溶性蛋白含量、POD活力提高。适宜浓度的PP333可以改变试管苗的生理特性,达到培育壮苗的目的。The effects of PP333 on form and physiological characteristics of Rehmannia glutinosa Libosch plantlets were studied. The effect of PP333 were explored by single factor design, colorimetry and Guaiacol process. The results showed that different concentration of PP333 could improve the buds pullulation, increase the numbers of adventitious roots and make them stronger. Lower concentrations of PP333 (0. 01, 0. 05mg·L^-1 ) accelerated the extension of stems but higher concentrations (0. 1, 2 mg·L^-1) inhibited the upgrowth of stems and leaves. PP333 (2 mg · L^-1) was suitable to produce strong plantlets. PP333 also increased content of soluble protein and activity of POD in leaves during middle time of upgrowth. Suitable concentration of PP333 can improve the physiological characteristics of Rehmannia glutinosa Libosch plantlets to produce strong ones.
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