金龟子绿僵菌MA4菌株蛋白酶Pr2基因的克隆及其表达分析  

Cloning and Characterization of the Neutral Pr2 Gene in Metarhizium Anisopliae MA4

作　　者：唐复润[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南省热带农业有害生物检测监控重点实验室,海南儋州571737

出　　处：《华南热带农业大学学报》2007年第1期1-4,共4页Journal of South China University of Tropical Agriculture

基　　金：科技部"国家科技基础条件平台工作"(编号:2005DKA30506);科技部"科技基础性工作和社会公益研究专项"(编号:2004DIB3J073)资助项目。

摘　　要：克隆了金龟子绿僵菌MA4菌株蛋白酶Pr2基因,编码序列长度为771bp,推导蛋白由256个氨基酸组成,是一疏水性蛋白。与菌株ME1得到Pr2编码序列(GeneBank/X78875,AJ242736)相比,MA4比菌株ME1(X78875)多6个核苷酸,同源性为97.4%,编码的氨基酸同源性为95.3%;MA4与菌株ME1(AJ242736)的核苷酸数目相同,同源性达到97.8%,编码的氨基酸同源性达98.1%。MA4菌株对东亚飞蝗4龄幼虫的LT50为4.83d,RT-PCR检测Pr2基因在东亚飞蝗体内的表达:接种第三天时扩增预期771bp条带。The Pr2 gene was cloned from isolation MA4 of metarhizium anisopliae. The fragment was 771bp in full length. It was deduced that a protein, composed of 256 amino acids, was a hydrophobicity protein. The nucleotide is closely identified with isolation ME1 （GeneBank/X78875, AJ242736） , and the isogenousness is 97.4%. The isogenousness of encoded amino acids is 95.3%. MA4 has the same number of nucleotide acids as ME1, with the isogenousness reaching 97.8%, and the isogenousness of encoded amino acids is 98.1%. When inoculated by isolation MA4 to locusts, the LT50 is 4.83 d. The Pr2 gene assayed by RT-PCT expressed in locusts： at 3 days, the 771 bp band was amplified.

关 键 词：绿僵菌 Pr2基因 蝗虫 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]