荧光法研究恩诺沙星与牛血清白蛋白的结合作用  

Studies on the Interaction between Enrofloxacin and Bovine Serum Albumin by Fluorescence

作　　者：张国文[1] 阙青民[1] 潘军辉[1] 胡勇克[2] 

机构地区：[1]南昌大学食品科学教育部重点实验室,江西南昌330047 [2]南昌大学资产管理处,江西南昌330031

出　　处：《南昌大学学报（理科版）》2007年第1期65-68,共4页Journal of Nanchang University(Natural Science)

基　　金：长江学者和创新团队发展计划资助项目(IRT0540);江西省教育厅科技计划资助项目(赣教技字[2005]-71号)

摘　　要：应用荧光光谱法、吸光光度法研究了生理条件下(pH=7.4)恩诺沙星(ENR)与牛血清白蛋白(BSA)的相互作用。实验表明,ENR对BSA内源性荧光的猝灭机制属于形成了复合物的单一静态猝灭过程,其结合常数为2.60×105L.mol-1,结合位点数为1.07。根据F rster非辐射能量转移理论,计算出恩诺沙星与BSA结合位置距色氨酸残基的距离为2.57 nm。能量转移效率为0.279。此外,探讨了共存Cu(Ⅱ)、Zn(Ⅱ)对药物与血清白蛋白结合作用的影响。The interaction between enrofloxacin and bovine serum albumin （BSA） in physiological condition was studied by fluorescence and spectrophotometry. The experiment demonstrated that the combination for enrofloxacin with BSA was a single static quenching process. The binding constant is 2.60 × 10^5 L · mol^-1 ,and the number of binding sites is 1.07. The binding distance and energy transfer efficiency between donor （BSA） and acceptor （ enrofloxacin） were obtained based on the theory of Ftirster spectroscopy energy transfer,their value are 2.57 nm and 0. 279, respectively. In addition, the competition reaction for bovine serum albumin between Cu （ Ⅱ ） ,Zn （ Ⅱ ） and enrofloxacin were discussed.

关 键 词：恩诺沙星 牛血清白蛋白 荧光光谱 金属离子 

分 类 号：O657.3[理学—分析化学]