常温体外循环心脏不停跳手术对TNF-α影响的临床研究  

The clinical study of the effect of TNF-α on beating heart surgery during normothermic cardiopulmonary bypass

作　　者：杜爱民[1] 张秀和[2] 李晓平[1] 

机构地区：[1]长春市儿童医院,吉林长春130051 [2]吉林大学中日联谊医院

出　　处：《中国实验诊断学》2007年第4期457-459,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨常温体外循环(CPB)心脏不停跳手术对血清肿瘤坏死因子α(TNF-α)含量的影响及意义。方法30例先天性房间隔缺损患者,随机分为观察组(n=15例)在常温心脏不停跳下手术;对照组(n=15例)在低温心脏停跳下手术。全组均在CPB前、结束即刻、结束后2 h、结束后24 h取静脉血样标本,运用酶联免疫吸附试验(ELISA)方法检测血清中TNF-α的含量。两组之间各检测点进行比较,并进行t检验,分析数值。结果CPB结束后两组TNF-α含量均升高,观察组TNF-α在CPB结束后各时点的升高幅度低于对照组(P<0.05)。结论常温心脏不停跳手术可以减少TNF-α的释放,从而减轻CPB术后炎性反应。Objective To expore the changes and its significance of serumtumor necrosis factor-α（TNF-α） on beating heart surgery during normothermic cardiopulmonary bypass（CPB）. Methods 30 patients with congenital atrial septal defect were divided into observation group（ n = 15） and control group（ n = 15） randomly. The observation group received surgery on beating heart with normothermia and the control group received operation on arrested heart with moderate hypothermia CPB. Blood samples were collected before CPB, immediately after CPB, 2 h after CPB,24 h after CPB in observation and control group . The serum TNF-αwas measured by ELISA（enzyme-linked immunesorbent assay）technique .The data was dealt with statistics and compared between the two groups. Results TNF-αin beth groups was increased after CPB, TNF-αin observation group was lower at each time paint after CPB than those in control group （ P 〈 0.05）. Conclusion Beating heart surgery during normothemaic cardiopulmonary bypass can decrease the release of TNF-α and alleviate the systemic inflammatory response.

关 键 词：常温 心脏不停跳手术 肿瘤坏死因子Α 体外循环 

分 类 号：R654.2[医药卫生—外科学]