乙型肝炎病毒表面大蛋白与HBV DNA检测的对比研究  

A comparison research of hepatitis B virus large surface protein with HBV DNA detecting

作　　者：吴正林[1] 刘玢 肖桂初 刘键[1] 林广城 吴意 陆学东[1] 

机构地区：[1]深圳市第四人民医院检验医学部,广东深圳518033

出　　处：《中国实验诊断学》2007年第4期479-481,共3页Chinese Journal of Laboratory Diagnosis

基　　金：深圳市福田区科技计划项目(FT200641)

摘　　要：目的探讨检测乙型肝炎病毒表面大蛋白(LHBs)用于临床乙型肝炎诊断的实验价值及与乙肝病毒DNA定量的相关性。方法采用酶联免疫吸附实验(ELISA)和荧光定量PCR法对600例HBsAg阳性血清标本进行LHBs及HBV DNA平行检测。结果①在600例HBsAg阳性血清中HBV DNA阳性率为76.17%(457/600),LHBs的阳性率为77.33%(464/600),二者无显著差异(χ2=0.696,P>0.05);②300份HBeAg阳性血清中,HBV DNA、LHBs阳性率分别为95.0%(285/300)9、6.0%(288/300);300份HBeAg阴性血清中,HBV DNA、LHBs阳性率分别为57.33%(172/300)、58.67%(176/300),二者差异均无统计学意义(χ2=0.725,P>0.05;χ2=0.253,P>0.05)。③LHBs含量与HBV DNA拷贝数呈正相关性,相关系数r=0.948。结论定量检测LHBs有助于了解乙型肝炎病毒复制情况。Objective To review the clinical value of hepatitis B virus large surface protein（LHBs）used for diagnosis of the clinical hepatitis B patient and rehtivity with hepatitis B virus DNA.Methods Enzyme Linked Immunosorbent Assay（ELISA） methods were used to examine the LHBs and quantitative real-time PCR methods were used to detect the HBV DNA of 600 HBsAg-positive serum samples.Results ①No significant difference of positive rate was observed between HBV DNA 76.17%（457/600） and LHBs 77.33 % （464/600） （Х^2 = 0.696, P 〉 0.05） in 600 HBsAg-positive serum samples. ② Posfive rate of HBV DNA and LHBs were 95.0%（285/300） and 96.0%（288/300） in 300 HBeAg postive samples and were 57.33%（172/300） and 58.67%（176/300） in 300 HBeAg negative samples（Х^2 = 0.725, P 〉 0.05; Х^2 = 0.253,P 〉 0.05） .③Semm LHBs levels were correlated with the serum HBV DNA copies （ r = 0.948）, Conclusion Our results demonstrated that the quantitative detecting of serum LHBs might be helpful to estimate the state of HBV DNA replication.

关 键 词：HBV 大蛋白 HBV DNA HBEAG 乙型肝炎病毒 

分 类 号：R450[医药卫生—治疗学]