维生素A缺乏对气管粘膜免疫的影响  

EFFECT OF VITAMIN A ON TRACHEAL MUCOSAL IMMUNITY

作　　者：陶月红[1] 杨毅[1] 王卫平[1] 

机构地区：[1]复旦大学附属儿科医院,上海200032

出　　处：《营养学报》2007年第2期134-137,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30371210)

摘　　要：目的探讨维生素A(VA)调节粘膜免疫的作用及其分子机制。方法建立维生素A缺乏(VAD)的大鼠模型,应用免疫组化、RT-荧光定量PCR和ELISA的方法,观察VA对气管粘膜树突状细胞数目(OX-62阳性细胞数)、成熟度(CD80、CCR7的阳性面积);抗原识别受体(TLR2和TLR4的阳性面积)和信号转导(MyD88的基因转录水平)及细胞因子产生的影响。结果VAD大鼠气管粘膜树突状细胞的数目显著增加,但成熟分化标志CD80和CCR7无明显变化。粘膜TLR2表达增强,MyD88mRNA水平上调。粘膜IL-12的表达升高,Th2细胞因子IL-4、IL-6的产生减少,IL-10的蛋白表达下调。结论VAD大鼠气管粘膜树突状细胞的数量、抗原识别受体水平和对细胞因子的调节都有显著变化,对树突状细胞的影响可能是VAD导致粘膜免疫功能下降的重要机制之一。Objective： To investigate the effects and mechanisms of vitamin A on tracheal mucosal immunity, Method： The rat model with vitamin A deficiency （VAD） was established. By use of immunohistochemical, RT-quantity PCR and ELISA methods, the number of dendritic cells （DCs, OX-62 positive cells）, the maturation of DCs （the positive areas of CD80 and CCR7）, the pathogen recognition receptors （the positive areas of TLR2 and of TLR4） and TLRs signal transduction （the mRNA level of MyD88）, as well as cytokines production in tracheal mucous membrane were detected. Results： In VAD rats, the number of DCs in trachea was increased, but the expressions of CD80 and CCR7 were similar with control group. The TLR2 and MyD88 mRNA levels were up-regulated, and IL-12 production was also raised. However, Th2 cytokines IL-4 and IL-6 were reduced significantly in protein and mRNA levels, and the production of IL-10 protein was declined compared with the control. Conclusion： In VAD rats, the number of DCs, receptors of pathogens recognition, and cytokines production in trachea mucosa were significantly changed. The affection of VAD on DCs may play important role in the impaired mucosal immunity of trachea.

关 键 词：维生素A 树突状细胞 细胞因子 TOLL样受体 粘膜免疫 

分 类 号：R56[医药卫生—呼吸系统]