EST-CAPS标记在尾叶桉和细叶桉遗传图谱构建中的应用  

Application of EST-CAPS Markers to Genetic Mapping in Eucalyptus urophylla and Eucalyptus tereticornis

作　　者：张照远[1] 甘四明[1] 李发根[1] 李梅[1] 翁启杰[1] 胡哲森[2] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]福建农林大学林学院,福建福州350002

出　　处：《林业科学研究》2007年第2期230-234,共5页Forest Research

基　　金：国家自然科学基金(30371173);863计划项目(2006AA100109);教育部"留学回国人员科研启动基金"

摘　　要：以已构建的尾叶桉和细叶桉RAPD连锁图谱为基础,利用CAPS技术对54个细叶桉EST序列在尾叶桉和细叶桉遗传图谱上的定位研究表明:7个EST序列在作图群体中呈等位片段多态性,包括母本尾叶桉特有的3个(其中1个偏分离)、父本细叶桉特有的2个和父母本共有的2个;共有4个EST-CAPS标记整合到尾叶桉RAPD连锁图谱,分散于不同的连锁群,各连锁群大小均有小幅增加;细叶桉RAPD连锁图谱上也整合了4个EST-CAPS标记,分散于不同的连锁群,各连锁群大小均有不同程度的增加;另外,尾叶桉上的1个偏分离标记未整合到任何连锁群。EST-CAPS可以有效用于桉属树种遗传图谱构建。Seven EST-CAPS markers were identified from 54 candidate ESTs for genetic mapping with an F, population of Eucalyptus urophylla x E. tereticornis, including three specific in female parent （ one being aberrant from normal segregation among the sibs）, two specific in male parent, and two shared by both parents. Four EST-CAPS markers were integrated to independent linkage groups on the RAPD linkage map of E. urophylla constructed previously, which contributed to some degrees to the extension of linkage group length. Also, four EST-CAPSs were mapped to different linkage groups on the RAPD linkage map of E. tereticornis, which gave rise to the increase of linkage group length. In addition, the aberrant marker was found to be unlinked to any linkage group in E. urophylla. This study demonstrated the usefulness of EST-CAPS markers in genetic mapping in Eucalyptus.

关 键 词：EST—CAPS 尾叶桉 细叶桉 遗传图谱 

分 类 号：S792.39[农业科学—林木遗传育种]