龙胆抗病毒有效部位RG2-1体外抗RSV作用的实验研究  

The anti-respiratory syncytial virus effect of an active compound RG2-1 from Radix Gentianae in vitro

作　　者：李鑫[1] 李洪源[1] 谢志平[1] 陈振华[1] 张建军[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081

出　　处：《疾病控制杂志》2007年第2期154-156,共3页Chinese Journal of Disease Control and Prevention

摘　　要：目的了解龙胆抗病毒有效部位RG2-1对呼吸道合胞病毒(RSV)的体外抑制作用。方法经水提取、二氯甲烷萃取、离心后,得到龙胆抗病毒有效部位RG2-1,并以利巴韦林为阳性对照药物,采用细胞病变抑制实验观察其在Hela细胞中对RSV的抑制作用。结果RG2-1的半数中毒浓度(TC50)为10.66mg/ml-1,抑制RSV的半数有效浓度(EC50)为0.42mg/ml,治疗指数(TI)为25.38;随着药物浓度的增加,CPE抑制率明显增高,且对RSV的抑制作用存在明显的量效反应关系(P<0.05)。结论RG2-1在Hela细胞中对RSV具有明显的抑制作用。Objective To observe the anti-respiratory syncytial virus （RSV） effect of an active compound RG2-1 from Radix Gentianae in vitro. Methods RG2-1 was extracted from Radix Gentianae by hot water extraction, dichloromethane extract and centrifugalization. The CPE inhibition assay was used to test the antiviral activity of RG2-1 on RSV in Hela cells while ribavirin was used as a positive control. Results RG2-1 was effective antiviral agent for RSV in a concentration-dependent manner. The median toxic concentration （TC50） was 10. 66 mg/ml, the median effective concentration （EC60） was 0.42 mg/ml and the treatment index （TI） was 25.38. Conclusions RG2-1 has an obvious anti-RSV effect in vitro.

关 键 词：龙胆 呼吸道合胞病毒 抗病毒药 

分 类 号：R373.14[医药卫生—病原生物学] R978.7[医药卫生—基础医学]