柚苷酶产生菌的抗药性UV诱变筛选  

A Study on the Screening of Chemical Resistance of Naringinase-producing Strains by UV Induction

作　　者：陈玲[1] 涂晓嵘[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《江西农业大学学报》2007年第2期297-300,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家创新基金项目(04C26213600633)

摘　　要：通过敏感抗生素对柚苷酶产生菌3-54菌株孢子致死浓度的测定,采用不同的UV照射时间对菌株孢子进行诱变处理,将诱变后的孢子涂在含纳他霉素致死浓度(2.0 U/mL)的平板筛选培养基上,获得了大量的抗药性致死突变株。从120个纳他霉素抗性基因突变株中通过透明圈初筛和多次摇瓶复筛,得到1株摇瓶发酵单位为500 U/mL的3-54-7号菌株,比出发菌株的产酶能力提高了近30%。After testing the resistance of sensitive antibiotic to Naringinase - producing strains No. 3 - 54, a lot of chemical resistant mutants were screened after the spores regenerated on the lethal media when they were treated with several different times of UV. A high -yield strain 3 -54 -7 was obtained from the 120 mutants,the productivity of which is 500 U/mL, almost 30% higher than that of the original strain in the rotation - flask experiment.

关 键 词：柚苷酶 抗药性 致死突变 

分 类 号：Q935[生物学—微生物学]