三氧化二砷对人肺腺癌A549/R细胞多药耐药相关蛋白表达的影响  

Effect of Arsenic Tioxide on MRP Expression in A549/R Cell Line of Human Lung Cancer

作　　者：成静[1] 冯觉平[2] 王亚萍[2] 李敏[2] 孔庆志[2] 

机构地区：[1]武汉大学中南医院肿瘤科,430071 [2]武汉市普爱医院肿瘤科,430030

出　　处：《医药导报》2007年第5期457-460,共4页Herald of Medicine

基　　金：武汉市卫生局基金资助项目(基金编号:[2003]228号)

摘　　要：目的探讨三氧化二砷(As2O3)对人肺腺癌A549/R细胞耐药性的逆转作用及对多药耐药相关蛋白(MRP)表达的影响。方法以荧光分光光度计测定细胞内药物浓度的改变,采用半定量逆转录聚合酶联反应(RT-PCR)技术检测As2O3处理后A549/R MRP基因表达的变化。结果As2O3的非细胞毒性剂量可增加A549/R细胞内多柔比星(ADM)浓度,降低其IC50。A549/R细胞中MRP呈过表达状态,不同浓度的As2O3处理A549/R后MRP表达水平明显降低。结论As2O3可部分逆转A549/R细胞对ADM的耐药性,其逆转机制与改变MRP基因表达有关。Objective To investigate the effect of arsenic trioxide （ As2 O3 ） on the reversal role of drug resistance and the expression of MRP in human lung cancer cell line A549/R . Methods The comcentration of intracellular ADM was detected by fluorescence spectrofluorometry. The expression of MRP gene was assessed in As2O3 -treated A549/R cells by semiq- uantitative RT-PCR. Results As2O3 in noncytotoxic dose could significantly increase the intracellular accumulation of ADM in A549/R cell line and derease the medium inhibition concentration （ IC50）. The overexpression of MRP mRNA was observed in A549/R cell line and this was significantly suppressed in As2 O3-treated A549/R cells. Conclusion As2O3 can partically reverse the resistance to ADM in A549/R cell line and this effect may be related to the variation of MRP gene expression.

关 键 词：三氧化二砷 人肺腺癌A549/R细胞 多药耐药 多药耐药相关蛋白 

分 类 号：R979.1[医药卫生—药品] R965.2[医药卫生—药学]