丹参酚酸B和姜黄素抑制大鼠肝星状细胞细胞外信号调节激酶的磷酸化  

Salvianolic Acid B and Curcumin Inhibit the Phosphorylation of Extracellular Signal Regulated Kinase in Rat Hepatic Stellate Cells

作　　者：成扬[1] 平键[1] 刘成[1] 谭英姿[1] 陈高峰[1] 

机构地区：[1]上海中医药大学附属曙光医院肝二科,上海201203

出　　处：《中国现代应用药学》2007年第2期96-100,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(No.30300458);上海市教委重点学科建设项目(Y0302)

摘　　要：目的研究丹参酚酸B(SAB)和姜黄素(Cur)对大鼠肝星状细胞(HSC)增殖、活化和细胞外信号调节激酶(ERK)的作用。方法培养大鼠HSC-T6,使用SAB和Cur处理细胞,采用MTT法检测药物对细胞增殖的影响。收集裂解细胞并抽提细胞总蛋白,10%聚丙烯酰胺凝胶电泳分离蛋白,采用Western blot法检测药物对细胞表达α平滑肌肌动蛋白(α-SMA)、I型胶原、ERK和磷酸化ERK的影响。结果Cur剂量依赖性地抑制大鼠HSC的增殖和活化,SAB和Cur显著降低α-SMA的表达水平(P<0.01);SAB对I型胶原的分泌无影响(P>0.05)而Cur显著减少I型胶原的分泌(P<0.05)。SAB和Cur对ERK表达水平都没有显著影响(P>0.05),但是显著降低了磷酸化ERK的表达水平(P<0.01 vs.P<0.05)。结论SAB和Cur能够抑制HSC的增殖、活化和ERK的磷酸化。OBJECTIVE To study the effect of Salvianolic acid B （SAB） and Curcumin on the proliferation, activation and extracellular signal regulated kinase （ERK） in rat hepatic stellate cells. METHODS Rat hepatic stellate cells （HSC） ,T6,were cultured and treated with SAB or Curcumin according to the experimental protocol. The inhibition effect on cell proliferation was determined by 3-（4,5-dimthyl-2-2thiazoly）-2,5-diphenyl-2H-tetrazolium bromide colorimetry. Cells were harvested and lysed to extract total cellular proteins. Proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the expression levels of ct smooth muscle actin （ct-SMA）, type Ⅰ collagen, ERK and phosphorylated-ERK after drug treatments were determined by Western blot. RESULTS Curcumin inhibited HSC proliferation in a dose-dependent manner. Both SAB and Curcumin reduced the expression level of α-SMA significantly （ P 〈 0.01 ）. SAB had no effect on the expression of type Ⅰ collagen （ P 〉 0.05 ） while Curcumin reduced the expression of type Ⅰ collagen significantly （ P 〈 0.05 ）. Both of SAB and Curcumin had no significant effect on the ERK expression level （P 〉 0.05）, but significantly reduced phosphorylated-ERK expression level （ P 〈 0.01 vs. P 〈 0.05, respectively）. CONCLUSION SAB and Curcumin could significantly inhibit the proliferation, activation and ERK phosphorylation.

关 键 词：丹参酚酸B 姜黄素 细胞外信号调节激酶 磷酸化 

分 类 号：R965.1[医药卫生—药理学]