PNA-磁性纳米人工切断试剂定位水解切断DNA  

Synthesis of magnetic nanoparticle-based artificial cleavage reagent for sequence-specific hydrolysis of single stranded DNA

作　　者：杨旭石[1] 王锋[1] 沈鹤柏[1] 朱龙章[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2007年第2期57-60,共4页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市科委基金项目(0552nm037;05DZ19327)

摘　　要：制备了以二氧化硅包裹的磁性纳米粒子(MNPs)为载体,PNA(肽核酸)为识别系统,Ce(IV)/EDTA配合物为切割系统的PNA-磁性纳米切割试剂.采用TEM对制备的MNPs进行了表征,通过IP-RP-HPLC对切断产物进行了分析.结果表明,该PNA-磁性纳米人工切断试剂可以成功地结合目标DNA,并对其进行定位切断.此外,磁性纳米粒子的引入,使得切断产物的分析更加简单、快捷.A novel magnetic nanoparticles （MNPs）-based artificial cleavage reagent for single-stranded DNA （ssDNA） was synthesized by binding molecular scissor of Ce （ Ⅳ ）/EDTA complex on the surface of MNPs via sequence-recognizing moiety （PNA）. The average size of the MNPs was determined around 100 nm by TEM. Its cleavage ability for ssDNA was investigated by HPLC, which revealed that the target ssDNA could be sequence-specifically hydrolyzed. Furthemore, MNPs greatly facilitate the isolation of the cleaved product, showing good potential in gene studies.

关 键 词：PNA 磁性纳米粒子 CE DNA切断 

分 类 号：O648[理学—物理化学]