控制水稻剑叶形态相关性状的数量基因位点(QTL)的定位  

Locating quantitative trait loci affecting flag-leaf shape traits in rice(Oryza sativa L.)

作　　者：肖珂[1] 左海龙[1] 巩迎军[1] 张俊芝[1] 张永娟[1] 董彦君[1] 

机构地区：[1]上海师范大学生命与环境科学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2007年第2期66-70,共5页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市教委项目(05DZ25;06ZZ21);上海科委浦江人才项目(06PJ14074);国家948重大项目(2006-G1);国家航天育种工程项目(2006HT100013);上海市科委重大基础项目(05DJ14008)

摘　　要：水稻籽粒中一半以上的碳水化合物来自剑叶的光合作用，剑叶形态改良一直是水稻株型育种的一个重要目标．利用一个日本主要种植的粳稻品种越光（轮回亲本）和一个印度的籼稻品种Kasalath杂交产生的回交重组自交系群体（backcross recombinant inbred lines，BILs）对剑叶形态中的3个主要性状（剑叶长、叶宽以及其叶面积）进行了相关分析及其数量基因位点（quantitative trait loci，QTL）的定位．研究表明，控制剑叶形态的3个主要性状间存在极显著的正相关，并检测到影响3个性状的8个QTL，分布在第1，3，4，6条染色体上，贡献率介于4．94％～22．07％，其中第4染色体上C1016标记和第6染色体上C556标记附近的共有6个QTL，其两侧的紧密分子标记在水稻株型分子育种上具有一定应用价值．More than half of carbohydrate in grains comes from flag-leaf photosynthesis in rice （ Oryza sativa L, ）. Improving the shape of flag leaf is one of important objectives for plant-type breeding in rice. Correlations and QTL analysis on three main traits affecting flag - leaf shape （ flag - leaf length, FLL; flag - leaf width, FLW; flag-leaf area, FLA） in rice were conducted using 182 backcross recombinant inbred lines（BILs） derived from the backcross between a leading variety in Japan, Koshihikari （ japonica ）, as a recurrent parent, and an India rice variety, Kasalath （ indica ）, as a donor parent. The results of this study showed that there existed significantly positive correlations among the three traits studied. A total of eight QTLs controlling the three flag-leaf shape traits were detected and located on chromosomes 1,3,4 and 6, respectively, which could explain 4.94% 22.07% of total phenotypic variance. Among them, six QTLs detected in this study were located near to the common marker C1016 on chromosome 4 and C556 on chromosome 6, respectively, which indicated that the tightly linked molecular markers that flank the six QTLs will be valuable for plant-type improvement in rice through molecular breeding in future.

关 键 词：水稻 相关 剑叶形态 数量性状位点(QTL) 

分 类 号：Q943.2[生物学—植物学]