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机构地区:[1]泰山医学院附属医院检验科,山东泰安 271000
出 处:《国际检验医学杂志》2007年第4期340-341,344,共3页International Journal of Laboratory Medicine
摘 要:目的 对酮氨酸氧化酶法全自动测定糖化血清蛋白(GSP)的方法进行评价。方法 用日立7060全自动生化分析仪检测GSP,观察试剂稳定性、精密度、线性范围、抗干扰性、准确性、样品贮存温度和贮存时间,建立参考值范围。结果 该方法180s达到反应终点,线性达到1266μmol/L(Y=0.9866X+0.134,r=0.9097);批内CV为1.54%,批间CV为3.20%;平均回收率为101.7%;当TG〈9.8mmol/L、BIL〈675μmol/L、Hb〈200g/L、UA〈3500μmol/L、Glu〈100mmol/L时对测定结果无显著干扰。120例健康人血清GSP参考值范围(^-x±2s):110~220μmol/L。结论 本方法线性范围宽、特异性强、精密度高,干扰因素少,是测定GSP的较好方法。Objective To evaluate the methodological study of glycosylated serum proteins test (GSP) by the ketonization of amino-acid oxidase method. Methods The stability, imprecision,linearity, the interference response of this method,accuracy and storage temperature and storage time were observed at automated analyzer Hitachi 7060 for establishing the range of reference value. Results The end of reaction was at 180 sec. The linearity of method was up to 1 266 μmol/L(Y=0. 986 6X+ 0. 134, r=0. 999 7), the within-run coefficient of variation (CV) was 1.54%, the between-run CV was 3.20%, and the average recovery rates was 101. 7%. The assay wasn't significantly interfered when triglyceride(9. 8 mmol/L, bilirubin(675 μmol/L, hemoglobin(200 g/L, uric acid(3 500 μmol/L and glucose(100 mmol/L. GSP of serum samples from 120 healthy blood donors were determined and the reference value was 110-220 μmol/L. Conclusion This method has a characteristic of broad linearity, powerful specificity, high precision degree and less interfering factors. The ketonization of amino-acid oxidase method is an excellent measurement for GSP.
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