Site-specifically cleavage of oxidized insulin B chain with Zn(Ⅱ) ion studied by electrospray ionization mass spectrometry  

作　　者：Jiang Jiang Yu Hua Mei Long Gen Zhu Wen Ji Wang 

机构地区：[1]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, Chin [2]College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3]State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China

出　　处：《Chinese Chemical Letters》2007年第5期557-560,共4页中国化学快报（英文版）

摘　　要：The electrospray ionization mass spectrometry and tandem mass spectrometry investigation showed that the binding sites of Zn^2＋ with oxidized insulin B chain are His 5, His 10, and Arg 22, which lead to the selective cleavages of the peptide bonds at Ash 3- Gin 4, His 5-Leu 6, Gly 8-Ser 9, and Glu 21-Arg 22 of oxidized insulin B chain.The electrospray ionization mass spectrometry and tandem mass spectrometry investigation showed that the binding sites of Zn^2＋ with oxidized insulin B chain are His 5, His 10, and Arg 22, which lead to the selective cleavages of the peptide bonds at Ash 3- Gin 4, His 5-Leu 6, Gly 8-Ser 9, and Glu 21-Arg 22 of oxidized insulin B chain.

关 键 词：Oxidized insulin B chain ZNCL2 Mass spectrometry Hydrolytic cleavage 

分 类 号：Q578[生物学—生物化学]