新风胶囊对佐剂性关节炎大鼠肺部HRCT及血清细胞因子的影响  

Influence of Xinfeng Capsule on Pulmonary HRCT Images and Cytokines in Blood Serum of Adjuvant Arthritis Rats

作　　者：刘健[1] 范海霞[1] 杨梅云[1] 

机构地区：[1]安徽中医学院第一附属医院,安徽合肥230031

出　　处：《山西中医学院学报》2007年第2期8-10,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家中医药管理局专项课题(项目编号:04-05LP27);安徽省自然科学基金项目(项目编号:050431002)

摘　　要：目的:观察新风胶囊对佐剂性关节炎(adjuvantarthritis,AA)大鼠肺部高分辨率CT(highresolution ratio CT,HRCT)及血清细胞因子的影响。方法:将60只大鼠随机分为正常组、模型组、甲氨喋呤(methotrexate,MTX)组、雷公藤多苷片(tripterygium wilfordii polycoridetablet,TPT)组和新风胶囊(Xinfeng capsule,XFC)组,每组12只。将除正常组外的其余动物右后足跖皮内注射弗氏完全佐剂0.1mL造模,造模后第19天开始给药。正常组及模型组均给予生理盐水,其余3组分别给予MTX、TPT、XFC。观察各组大鼠肺部HRCT及血清细胞因子的变化。结果:HRCT表现为模型组大鼠肺轮廓欠清晰,肺部血管影扩张,边缘模糊且不规则,两肺有索条状密度增高影,邻近胸膜轻度肥厚;甲氨喋呤组大鼠肺部可见沿着肺纹理分布的斑片状密度增高影,肺血管纹理模糊,边缘不清楚;雷公藤多苷片组大鼠两肺可见多发性结节状及斑片状密度增高影,境界欠清晰;新风胶囊组大鼠肺轮廓清晰,肺纹理分布均匀,部分肺组织显示斑点状密度增高影。与正常组比较,模型组大鼠血清中TNF-α显著升高(P<0.05),IL-10显著降低(P<0.01);与模型组比较,3个给药组大鼠血清中TNF-α含量显著降低(P<0.01);IL-10含量显著升高(P<0.01),但3给药组之间无显著性差异。结论:新风胶囊能改善AA大鼠肺部影像学表现、降低AA大鼠足跖肿胀度。其机制可能与降低致炎因子TNF-α含量、升高抗炎因子IL-10含量有关。Objective：To observe the influence of Xinfeng capsule （XFC）on pulmonary H RCT images and cytokines （TNF-α,IL-10）in blood serum of adjuvant arthritis rats. Method：60 rats were randomly divided into normal group,model group, methotrexate（MTX）treatment group,tripterygium wilfordii polycoride tablet（TPT） treatment group,XFC treatment group（n= 12）. Except for normal group,the rats of other groups were intracutaneously injected with 0.1 mL of Freund＇s complete adjuvant in the right hindlimb,and treatment drugs were given to corresponding rats 19 days later. The pulmonary HRCT images and the cytokines in blood serum of rats were observed. Results：The lungs＇ outlines of model group rats weren＇t clear,the blood vessels expanded and their edges were obscure and unregular. There were high density images as rope in lungs and a little thickening near pleura of model group rats. There were high density images shaped as slice along pulmonary veins,the veins of the blood vessels were obscure and the edges were unclear in the lungs of MTX treatment group rats. There were multiple high density images shaped as tubercle and slice and the outlines were not clear in the lungs of TPT treatment group rats. In XFC treatment group rats,the lungs＇ outlines were clear,the veins of lungs were well-distributed and high density images shaped as spot were found in part lungs. Compared with normal group rats,the TNF-α content in blood serum of model group rats ascended obviously （P〈0.05）,but the IL-10 content lowered obviously （P〈0.01）. Compared with model group rats,the TNF-α contents in blood serum of 3 treatment groups lowered obviously （P〈0.01） and IL-10 contents ascended obviously （P〈0.01）, but there were no obvious differences among 3 treatment groups（P〉0.05）. Conclusion：XFC can improve the pulmanory HRCT images manifestation of adjuvant arthritis rats and eliminates swelling of rats＇ right hindlimb. The mechanism maybe relate to up-regulating the content of TNF-α and

关 键 词：佐剂性关节炎 HRCT 细胞因子 新风胶囊 

分 类 号：R684.3[医药卫生—骨科学] R593.22[医药卫生—外科学]